Genetic diversity in wild barley (Hordeum spontaneum C. Koch) in the Near East: A molecular analysis using Random Amplified Polymorphic DNA (RAPD) markers

Bernard R. Baum, E. Nevo, Douglas A. Johnson, A. Beiles

Research output: Contribution to journalArticlepeer-review

Abstract

We analyzed the genetic diversity in 88 genotypes from 20 populations of wild barley (Hordeum spontaneum C. Koch) from Israel, Turkey and Iran, by randomly amplified polymorphic DNA (RAPD). Twenty two of the 33 primers used yielded scorable products with 1-11 polymorphic bands. No duplicate patterns were found except for four haplotypes. When the total genetic diversity was estimated, 75% of the variation detected was partitioned within the 88 genotypes and 25% among the populations. When variation between countries was assessed, no substantial differences were found, because most of the variation detected (97%) was partitioned within the 20 populations and the remainder among countries. The results of this limited survey indicate that the extensive genetic diversity is present in natural stands of wild barley throughout the Fertile Crescent.

Original languageEnglish
Pages (from-to)147-157
Number of pages11
JournalGenetic Resources and Crop Evolution
Volume44
Issue number2
DOIs
StatePublished - 1997

Bibliographical note

Funding Information:
Mr. A. Soueid, University of Ottawa, is gratefully acknowleged for his technical assistance. We are grateful to an anonymous referee for many helpful comments. This work was supported, in part, by a grant to D.A.J. from the Natural Sciences and Engineering Research Council of Canada.

Keywords

  • Genetic polymorphism
  • Hordeum spontaneum
  • wild barley

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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