Bibliographical noteFunding Information:
S.E. Fryer, C.J. Bayne Department of Zoology, Oregon State University, Corvallis, OR 97331, USA The gastropod mollusc Biomphalaria glabrata serves as intermediate host of the human trematode Schistosoma mansoni. Laboratory strains of this mollusc with known susceptibility or resistance to the parasite have been studied extensively to determine the molecular bases of hostparasite compatibility. Evidence exists for the involvement of component(s) in resistant plasma that allow normally benign susceptible hemocytes to recognize and kill parasite larvae. We have used latex microspheres to test the ability of the phagocytic hemocytes to distinguish between plasma components from susceptible and resistant snails. Hemocytes from susceptible snails phagocytosed negatively charged latex beads coated in resistant snail plasma at higher rates than those treated in plasma from susceptible strains. Susceptible hemocytes pretreated with plasma from resistant snails lost their ability to distinguish between such beads, suggesting recognition of a component unique to the resistant plasma. No differences in uptake by resistant hemocytes were seen. Lectin-carbohydrate interactions are thought to play a major role in non-self recognition in invertebrates. We therefore screened plasma-treated microspheres for both carbohydrate epitopes and endogenous lectins. Fluorochrome labelled lectins and neoglycoproteins were used to probe these plasma-treated particles. No interstrain differences were seen in the binding of a panel of 6 lectins. Differential binding of 3 neoglycoproteins indicates a difference in the endogenous lectins present in plasma from these two strains of B. glabrata. These lectins have a potential role in determining the outcome of host parasite interactions. Supported by NIH grant AI-16137.
ASJC Scopus subject areas
- Developmental Biology