Fungal substances as modulators of NF-κB activation pathway

Roumyana D. Petrova, Jamal Mahajna, Abraham Z. Reznick, Solomon P. Wasser, Cvetomir M. Denchev, Eviatar Nevo

Research output: Contribution to journalArticlepeer-review

Abstract

MCF7 breast cancer cell line, carrying a luciferase reporter gene under the control of nuclear factor kappa B (NF-κB)-responsive promoter, was established and used for the screening of fungal organic extracts for their ability to interfere with the NF-κB activation pathway. Twenty-eight crude fungal extracts, out of 242, were found to inhibit NF-κB reporter activity by more than 40%. Furthermore, positive extracts were used to evaluate their antiproliferative activity as well as their ability to influence the phosphorylation and degradation levels of IκBa. Fungal extracts prepared from Marasmius oreades and Cyathus striatus showed significant inhibitory effects on the NF-κB activation pathway. Taken together, our results support the notion of the presence of novel activities that might be utilized as cancer therapeutics.

Original languageEnglish
Pages (from-to)145-154
Number of pages10
JournalMolecular Biology Reports
Volume34
Issue number3
DOIs
StatePublished - Sep 2007

Bibliographical note

Funding Information:
Acknowledgments We thank Dr. J. Duyster, Department of Internal Medicine III, Technical University of Munich, Munich, Germany, for providing the Baf3/p185 Bcr-Abl cell line. We also thank Novartis Pharmaceuticals, Basel, Switzerland, for providing the Imatinib mesylate (Gleevec). We thank Mrs. Robin Permut for the English editing of the paper. This research was partly supported by the Ministry of Science and Technology of Israel, Grant No. alona5246 to J.M.

Keywords

  • Fungal substances
  • IκBα degradation
  • IκBα phosphorylation
  • NF-κB activation pathway

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

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