Functional characterization of powdery mildew resistance gene MlIW172, a new Pm60 allele and its allelic variation in wild emmer wheat

Qiuhong Wu; Yongxing Chen; Beibei Li; Jing Li; Panpan Zhang; Jingzhong Xie; Huaizhi Zhang; Guanghao Guo; Ping Lu; Miaomiao Li; Keyu Zhu; Wenling Li; Tzion Fahima; Eviatar Nevo; Hongjie Li; Lingli Dong; Zhiyong Liu

doi:10.1016/j.jgg.2022.01.010

Functional characterization of powdery mildew resistance gene MlIW172, a new Pm60 allele and its allelic variation in wild emmer wheat

Qiuhong Wu, Yongxing Chen, Beibei Li, Jing Li, Panpan Zhang, Jingzhong Xie, Huaizhi Zhang, Guanghao Guo, Ping Lu, Miaomiao Li, Keyu Zhu, Wenling Li, Tzion Fahima, Eviatar Nevo, Hongjie Li, Lingli Dong, Zhiyong Liu

Department of Evolutionary and Environmental Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Wild emmer wheat (Triticum dicoccoides, WEW) is an immediate progenitor of both the cultivated tetraploid and hexaploid wheats and it harbors rich genetic diversity against powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt). A powdery mildew resistance gene MlIW172 originated from WEW accession IW172 (G-797-M) was fine mapped in a 0.048 centimorgan (cM) genetic interval on 7AL, corresponding to a genomic region spanning 233 kb, 1 Mb and 800 kb in Chinese Spring, WEW Zavitan, and T. urartu G1812, respectively. MlIW172 was found to encode a typical NLR protein NLR^IW172 and physically located in an NBS-LRR gene cluster. NLR^IW172 was subsequently identified as a new allele of Pm60, and its function was validated by EMS mutagenesis and transgenic complementation. Haplotype analysis of the Pm60 alleles revealed diversifications in sequence variation in the locus and PAV (presence and absence variations) in WEW populations. Four common single nucleotide variations (SNV) were detected between the Pm60 alleles from WEW and T. urartu, indicative of speciation divergence between the two different wheat progenitors. The newly identified Pm60 alleles and haplotypes in WEW are anticipated to be valuable for breeding powdery mildew resistance wheat cultivars via marker-assisted selection.

Original language	English
Journal	Journal of Genetics and Genomics
DOIs	https://doi.org/10.1016/j.jgg.2022.01.010
State	Accepted/In press - 2022

Bibliographical note

Funding Information:
We are grateful to Profs. Qixin Sun and Tsomin Yang, China Agricultural University, China, for their advice and support during the research. Many thanks to Profs. Zhengqiang Ma and Haiyan Jia of Nanjing Agricultural University, China, for information exchange. This work was financially supported by National Science Foundation of China (31971876, U21A20224) and Scientific Research Project of Beijing Municipal Commission of Education (KM201910020014).

Funding Information:
We are grateful to Profs. Qixin Sun and Tsomin Yang, China Agricultural University, China , for their advice and support during the research. Many thanks to Profs. Zhengqiang Ma and Haiyan Jia of Nanjing Agricultural University, China, for information exchange. This work was financially supported by National Science Foundation of China ( 31971876 , U21A20224 ) and Scientific Research Project of Beijing Municipal Commission of Education ( KM201910020014 ).

Publisher Copyright:
© 2022 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China

Keywords

Allelic variation
Blumeria graminis f. sp. tritici
Pm60
Presence and absence variation
Triticum dicoccoides

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1016/j.jgg.2022.01.010

Cite this

Wu, Q., Chen, Y., Li, B., Li, J., Zhang, P., Xie, J., Zhang, H., Guo, G., Lu, P., Li, M., Zhu, K., Li, W., Fahima, T., Nevo, E., Li, H., Dong, L., & Liu, Z. (Accepted/In press). Functional characterization of powdery mildew resistance gene MlIW172, a new Pm60 allele and its allelic variation in wild emmer wheat. Journal of Genetics and Genomics. https://doi.org/10.1016/j.jgg.2022.01.010

@article{62f0dd2c71414f2ea1924ead8b92dd51,

title = "Functional characterization of powdery mildew resistance gene MlIW172, a new Pm60 allele and its allelic variation in wild emmer wheat",

abstract = "Wild emmer wheat (Triticum dicoccoides, WEW) is an immediate progenitor of both the cultivated tetraploid and hexaploid wheats and it harbors rich genetic diversity against powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt). A powdery mildew resistance gene MlIW172 originated from WEW accession IW172 (G-797-M) was fine mapped in a 0.048 centimorgan (cM) genetic interval on 7AL, corresponding to a genomic region spanning 233 kb, 1 Mb and 800 kb in Chinese Spring, WEW Zavitan, and T. urartu G1812, respectively. MlIW172 was found to encode a typical NLR protein NLRIW172 and physically located in an NBS-LRR gene cluster. NLRIW172 was subsequently identified as a new allele of Pm60, and its function was validated by EMS mutagenesis and transgenic complementation. Haplotype analysis of the Pm60 alleles revealed diversifications in sequence variation in the locus and PAV (presence and absence variations) in WEW populations. Four common single nucleotide variations (SNV) were detected between the Pm60 alleles from WEW and T. urartu, indicative of speciation divergence between the two different wheat progenitors. The newly identified Pm60 alleles and haplotypes in WEW are anticipated to be valuable for breeding powdery mildew resistance wheat cultivars via marker-assisted selection.",

keywords = "Allelic variation, Blumeria graminis f. sp. tritici, Pm60, Presence and absence variation, Triticum dicoccoides",

author = "Qiuhong Wu and Yongxing Chen and Beibei Li and Jing Li and Panpan Zhang and Jingzhong Xie and Huaizhi Zhang and Guanghao Guo and Ping Lu and Miaomiao Li and Keyu Zhu and Wenling Li and Tzion Fahima and Eviatar Nevo and Hongjie Li and Lingli Dong and Zhiyong Liu",

note = "Funding Information: We are grateful to Profs. Qixin Sun and Tsomin Yang, China Agricultural University, China, for their advice and support during the research. Many thanks to Profs. Zhengqiang Ma and Haiyan Jia of Nanjing Agricultural University, China, for information exchange. This work was financially supported by National Science Foundation of China (31971876, U21A20224) and Scientific Research Project of Beijing Municipal Commission of Education (KM201910020014). Funding Information: We are grateful to Profs. Qixin Sun and Tsomin Yang, China Agricultural University, China , for their advice and support during the research. Many thanks to Profs. Zhengqiang Ma and Haiyan Jia of Nanjing Agricultural University, China, for information exchange. This work was financially supported by National Science Foundation of China ( 31971876 , U21A20224 ) and Scientific Research Project of Beijing Municipal Commission of Education ( KM201910020014 ). Publisher Copyright: {\textcopyright} 2022 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China",

year = "2022",

doi = "10.1016/j.jgg.2022.01.010",

language = "English",

journal = "Journal of Genetics and Genomics",

issn = "1673-8527",

publisher = "Institute of Genetics and Developmental Biology",

}

TY - JOUR

T1 - Functional characterization of powdery mildew resistance gene MlIW172, a new Pm60 allele and its allelic variation in wild emmer wheat

AU - Wu, Qiuhong

AU - Chen, Yongxing

AU - Li, Beibei

AU - Li, Jing

AU - Zhang, Panpan

AU - Xie, Jingzhong

AU - Zhang, Huaizhi

AU - Guo, Guanghao

AU - Lu, Ping

AU - Li, Miaomiao

AU - Zhu, Keyu

AU - Li, Wenling

AU - Fahima, Tzion

AU - Nevo, Eviatar

AU - Li, Hongjie

AU - Dong, Lingli

AU - Liu, Zhiyong

N1 - Funding Information: We are grateful to Profs. Qixin Sun and Tsomin Yang, China Agricultural University, China, for their advice and support during the research. Many thanks to Profs. Zhengqiang Ma and Haiyan Jia of Nanjing Agricultural University, China, for information exchange. This work was financially supported by National Science Foundation of China (31971876, U21A20224) and Scientific Research Project of Beijing Municipal Commission of Education (KM201910020014). Funding Information: We are grateful to Profs. Qixin Sun and Tsomin Yang, China Agricultural University, China , for their advice and support during the research. Many thanks to Profs. Zhengqiang Ma and Haiyan Jia of Nanjing Agricultural University, China, for information exchange. This work was financially supported by National Science Foundation of China ( 31971876 , U21A20224 ) and Scientific Research Project of Beijing Municipal Commission of Education ( KM201910020014 ). Publisher Copyright: © 2022 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China

PY - 2022

Y1 - 2022

N2 - Wild emmer wheat (Triticum dicoccoides, WEW) is an immediate progenitor of both the cultivated tetraploid and hexaploid wheats and it harbors rich genetic diversity against powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt). A powdery mildew resistance gene MlIW172 originated from WEW accession IW172 (G-797-M) was fine mapped in a 0.048 centimorgan (cM) genetic interval on 7AL, corresponding to a genomic region spanning 233 kb, 1 Mb and 800 kb in Chinese Spring, WEW Zavitan, and T. urartu G1812, respectively. MlIW172 was found to encode a typical NLR protein NLRIW172 and physically located in an NBS-LRR gene cluster. NLRIW172 was subsequently identified as a new allele of Pm60, and its function was validated by EMS mutagenesis and transgenic complementation. Haplotype analysis of the Pm60 alleles revealed diversifications in sequence variation in the locus and PAV (presence and absence variations) in WEW populations. Four common single nucleotide variations (SNV) were detected between the Pm60 alleles from WEW and T. urartu, indicative of speciation divergence between the two different wheat progenitors. The newly identified Pm60 alleles and haplotypes in WEW are anticipated to be valuable for breeding powdery mildew resistance wheat cultivars via marker-assisted selection.

AB - Wild emmer wheat (Triticum dicoccoides, WEW) is an immediate progenitor of both the cultivated tetraploid and hexaploid wheats and it harbors rich genetic diversity against powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt). A powdery mildew resistance gene MlIW172 originated from WEW accession IW172 (G-797-M) was fine mapped in a 0.048 centimorgan (cM) genetic interval on 7AL, corresponding to a genomic region spanning 233 kb, 1 Mb and 800 kb in Chinese Spring, WEW Zavitan, and T. urartu G1812, respectively. MlIW172 was found to encode a typical NLR protein NLRIW172 and physically located in an NBS-LRR gene cluster. NLRIW172 was subsequently identified as a new allele of Pm60, and its function was validated by EMS mutagenesis and transgenic complementation. Haplotype analysis of the Pm60 alleles revealed diversifications in sequence variation in the locus and PAV (presence and absence variations) in WEW populations. Four common single nucleotide variations (SNV) were detected between the Pm60 alleles from WEW and T. urartu, indicative of speciation divergence between the two different wheat progenitors. The newly identified Pm60 alleles and haplotypes in WEW are anticipated to be valuable for breeding powdery mildew resistance wheat cultivars via marker-assisted selection.

KW - Allelic variation

KW - Blumeria graminis f. sp. tritici

KW - Pm60

KW - Presence and absence variation

KW - Triticum dicoccoides

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U2 - 10.1016/j.jgg.2022.01.010

DO - 10.1016/j.jgg.2022.01.010

M3 - Article

C2 - 35167980

AN - SCOPUS:85127793713

JO - Journal of Genetics and Genomics

JF - Journal of Genetics and Genomics

SN - 1673-8527

ER -

Functional characterization of powdery mildew resistance gene MlIW172, a new Pm60 allele and its allelic variation in wild emmer wheat

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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