Functional characterization of powdery mildew resistance gene MlIW172, a new Pm60 allele and its allelic variation in wild emmer wheat

Qiuhong Wu, Yongxing Chen, Beibei Li, Jing Li, Panpan Zhang, Jingzhong Xie, Huaizhi Zhang, Guanghao Guo, Ping Lu, Miaomiao Li, Keyu Zhu, Wenling Li, Tzion Fahima, Eviatar Nevo, Hongjie Li, Lingli Dong, Zhiyong Liu

Research output: Contribution to journalArticlepeer-review

Abstract

Wild emmer wheat (Triticum dicoccoides, WEW) is an immediate progenitor of both the cultivated tetraploid and hexaploid wheats and it harbors rich genetic diversity against powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt). A powdery mildew resistance gene MlIW172 originated from WEW accession IW172 (G-797-M) was fine mapped in a 0.048 centimorgan (cM) genetic interval on 7AL, corresponding to a genomic region spanning 233 kb, 1 Mb and 800 kb in Chinese Spring, WEW Zavitan, and T. urartu G1812, respectively. MlIW172 was found to encode a typical NLR protein NLRIW172 and physically located in an NBS-LRR gene cluster. NLRIW172 was subsequently identified as a new allele of Pm60, and its function was validated by EMS mutagenesis and transgenic complementation. Haplotype analysis of the Pm60 alleles revealed diversifications in sequence variation in the locus and PAV (presence and absence variations) in WEW populations. Four common single nucleotide variations (SNV) were detected between the Pm60 alleles from WEW and T. urartu, indicative of speciation divergence between the two different wheat progenitors. The newly identified Pm60 alleles and haplotypes in WEW are anticipated to be valuable for breeding powdery mildew resistance wheat cultivars via marker-assisted selection.

Original languageEnglish
JournalJournal of Genetics and Genomics
DOIs
StateAccepted/In press - 2022

Bibliographical note

Funding Information:
We are grateful to Profs. Qixin Sun and Tsomin Yang, China Agricultural University, China, for their advice and support during the research. Many thanks to Profs. Zhengqiang Ma and Haiyan Jia of Nanjing Agricultural University, China, for information exchange. This work was financially supported by National Science Foundation of China (31971876, U21A20224) and Scientific Research Project of Beijing Municipal Commission of Education (KM201910020014).

Funding Information:
We are grateful to Profs. Qixin Sun and Tsomin Yang, China Agricultural University, China , for their advice and support during the research. Many thanks to Profs. Zhengqiang Ma and Haiyan Jia of Nanjing Agricultural University, China, for information exchange. This work was financially supported by National Science Foundation of China ( 31971876 , U21A20224 ) and Scientific Research Project of Beijing Municipal Commission of Education ( KM201910020014 ).

Publisher Copyright:
© 2022 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China

Keywords

  • Allelic variation
  • Blumeria graminis f. sp. tritici
  • Pm60
  • Presence and absence variation
  • Triticum dicoccoides

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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