Field tests for pollen viability; A comparative approach

David H. Firmage, Amots Dafni

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


Many tests for pollen viability have been proposed. The ability to determine this reproductive state in the field can be very important in botanical research as well as for horticultural purposes. After reviewing a large number of tests, we compared four methods of determining pollen viability. Each of these methods can easily be carried out in the field. Viability tests were carried out on 17 species from Israel and were compared to in vitro germination trials. No one test worked well on all species. The MTT stain was determined as the most effective for a wide number of species, but tests for individual species should be conducted before final determinations are made.

Original languageEnglish
Title of host publicationVIII International Symposium on Pollination - Pollination
Subtitle of host publicationIntegrator of Crops and Native Plant Systems
PublisherInternational Society for Horticultural Science
Number of pages8
ISBN (Print)9789066057654
StatePublished - 2001

Publication series

NameActa Horticulturae
ISSN (Print)0567-7572


  • Comparison
  • Isatin
  • Israel
  • Pollen viability
  • Stain-MTT
  • X-Gal

ASJC Scopus subject areas

  • Horticulture


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