Fear memory formation involves p190 RhoGAP and ROCK proteins through a GRB2-mediated complex

Raphael Lamprecht; Claudia R. Farb; Joseph E. LeDoux

doi:10.1016/S0896-6273(02)01047-4

Fear memory formation involves p190 RhoGAP and ROCK proteins through a GRB2-mediated complex

Raphael Lamprecht, Claudia R. Farb, Joseph E. LeDoux

Research output: Contribution to journal › Article › peer-review

Abstract

We used fear conditioning, which is known to alter synaptic efficacy in lateral amygdala (LA), to study molecular mechanisms underlying long-term memory. Following fear conditioning, the tyrosine phosphorylated protein p190 RhoGAP becomes associated with GRB2 in LA significantly more in conditioned than in control rats. RasGAP and Shc were also found to associate with GRB2 in LA significantly more in the conditioned animals. Inhibition of the p190 RhoGAP-downstream kinase ROCK in LA during fear conditioning impaired long- but not short-term memory. Thus, the p190 RhoGAP/ROCK pathway, which regulates the morphology of dendrites and axons during neural development, plays a central role, through a GRB2-mediated molecular complex, in fear memory formation in the lateral amygdala.

Original language	English
Pages (from-to)	727-738
Number of pages	12
Journal	Neuron
Volume	36
Issue number	4
DOIs	https://doi.org/10.1016/S0896-6273(02)01047-4
State	Published - 14 Nov 2002
Externally published	Yes

Bibliographical note

Funding Information:
We thank Keren Paz for excellent advice and discussions. This work was supported by NIH grants MH58911, MH46516, MH38774, MH00956, and a Human Frontier Science Program postdoctoral fellowship.

ASJC Scopus subject areas

General Neuroscience

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10.1016/S0896-6273(02)01047-4

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title = "Fear memory formation involves p190 RhoGAP and ROCK proteins through a GRB2-mediated complex",

abstract = "We used fear conditioning, which is known to alter synaptic efficacy in lateral amygdala (LA), to study molecular mechanisms underlying long-term memory. Following fear conditioning, the tyrosine phosphorylated protein p190 RhoGAP becomes associated with GRB2 in LA significantly more in conditioned than in control rats. RasGAP and Shc were also found to associate with GRB2 in LA significantly more in the conditioned animals. Inhibition of the p190 RhoGAP-downstream kinase ROCK in LA during fear conditioning impaired long- but not short-term memory. Thus, the p190 RhoGAP/ROCK pathway, which regulates the morphology of dendrites and axons during neural development, plays a central role, through a GRB2-mediated molecular complex, in fear memory formation in the lateral amygdala.",

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note = "Funding Information: We thank Keren Paz for excellent advice and discussions. This work was supported by NIH grants MH58911, MH46516, MH38774, MH00956, and a Human Frontier Science Program postdoctoral fellowship.",

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AU - Lamprecht, Raphael

AU - Farb, Claudia R.

AU - LeDoux, Joseph E.

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N2 - We used fear conditioning, which is known to alter synaptic efficacy in lateral amygdala (LA), to study molecular mechanisms underlying long-term memory. Following fear conditioning, the tyrosine phosphorylated protein p190 RhoGAP becomes associated with GRB2 in LA significantly more in conditioned than in control rats. RasGAP and Shc were also found to associate with GRB2 in LA significantly more in the conditioned animals. Inhibition of the p190 RhoGAP-downstream kinase ROCK in LA during fear conditioning impaired long- but not short-term memory. Thus, the p190 RhoGAP/ROCK pathway, which regulates the morphology of dendrites and axons during neural development, plays a central role, through a GRB2-mediated molecular complex, in fear memory formation in the lateral amygdala.

AB - We used fear conditioning, which is known to alter synaptic efficacy in lateral amygdala (LA), to study molecular mechanisms underlying long-term memory. Following fear conditioning, the tyrosine phosphorylated protein p190 RhoGAP becomes associated with GRB2 in LA significantly more in conditioned than in control rats. RasGAP and Shc were also found to associate with GRB2 in LA significantly more in the conditioned animals. Inhibition of the p190 RhoGAP-downstream kinase ROCK in LA during fear conditioning impaired long- but not short-term memory. Thus, the p190 RhoGAP/ROCK pathway, which regulates the morphology of dendrites and axons during neural development, plays a central role, through a GRB2-mediated molecular complex, in fear memory formation in the lateral amygdala.

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Fear memory formation involves p190 RhoGAP and ROCK proteins through a GRB2-mediated complex

Abstract

Bibliographical note

ASJC Scopus subject areas

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