Facilitated enumeration of the silicate bacterium Paenibacillus mucilaginosus comb. nov. (formerly Bacillus mucilaginosus) via tetrazolium chloride incorporation into a double agar-based solid growth medium

Annie Vardanian; Eyal Kurzbaum; Yair Farber; Monica Butnariu; Robert Armon

doi:10.1007/s12223-017-0567-y

Facilitated enumeration of the silicate bacterium Paenibacillus mucilaginosus comb. nov. (formerly Bacillus mucilaginosus) via tetrazolium chloride incorporation into a double agar-based solid growth medium

Annie Vardanian, Eyal Kurzbaum, Yair Farber, Monica Butnariu, Robert Armon

Department of Geography and Environmental Studies

Research output: Contribution to journal › Article › peer-review

Abstract

Accurate enumeration of Paenibacillus mucilaginosus (formerly Bacillus mucilaginosus) bacterium from environmental samples on solid medium is challenging owing to its extensive extracellular polysaccharides (EPS) excretion. In the present study, P. mucilaginosus enumeration has been facilitated by a simple modification: addition of triphenyl tetrazolium chloride (TTC) to growth medium and application of a second soft agar layer. Results show distinctively better and accurate colonies’ count. This method can be applied to all bacterial species that produce excessive EPS that may interfere with direct count.

Original language	English
Pages (from-to)	401-404
Number of pages	4
Journal	Folia Microbiologica
Volume	63
Issue number	3
DOIs	https://doi.org/10.1007/s12223-017-0567-y
State	Published - 1 May 2018

Bibliographical note

Publisher Copyright:
© 2017, Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i.

ASJC Scopus subject areas

Microbiology

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10.1007/s12223-017-0567-y

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title = "Facilitated enumeration of the silicate bacterium Paenibacillus mucilaginosus comb. nov. (formerly Bacillus mucilaginosus) via tetrazolium chloride incorporation into a double agar-based solid growth medium",

abstract = "Accurate enumeration of Paenibacillus mucilaginosus (formerly Bacillus mucilaginosus) bacterium from environmental samples on solid medium is challenging owing to its extensive extracellular polysaccharides (EPS) excretion. In the present study, P. mucilaginosus enumeration has been facilitated by a simple modification: addition of triphenyl tetrazolium chloride (TTC) to growth medium and application of a second soft agar layer. Results show distinctively better and accurate colonies{\textquoteright} count. This method can be applied to all bacterial species that produce excessive EPS that may interfere with direct count.",

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doi = "10.1007/s12223-017-0567-y",

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Facilitated enumeration of the silicate bacterium Paenibacillus mucilaginosus comb. nov. (formerly Bacillus mucilaginosus) via tetrazolium chloride incorporation into a double agar-based solid growth medium. / Vardanian, Annie; Kurzbaum, Eyal; Farber, Yair et al.
In: Folia Microbiologica, Vol. 63, No. 3, 01.05.2018, p. 401-404.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Facilitated enumeration of the silicate bacterium Paenibacillus mucilaginosus comb. nov. (formerly Bacillus mucilaginosus) via tetrazolium chloride incorporation into a double agar-based solid growth medium

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AU - Kurzbaum, Eyal

AU - Farber, Yair

AU - Butnariu, Monica

AU - Armon, Robert

PY - 2018/5/1

Y1 - 2018/5/1

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AB - Accurate enumeration of Paenibacillus mucilaginosus (formerly Bacillus mucilaginosus) bacterium from environmental samples on solid medium is challenging owing to its extensive extracellular polysaccharides (EPS) excretion. In the present study, P. mucilaginosus enumeration has been facilitated by a simple modification: addition of triphenyl tetrazolium chloride (TTC) to growth medium and application of a second soft agar layer. Results show distinctively better and accurate colonies’ count. This method can be applied to all bacterial species that produce excessive EPS that may interfere with direct count.

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Facilitated enumeration of the silicate bacterium Paenibacillus mucilaginosus comb. nov. (formerly Bacillus mucilaginosus) via tetrazolium chloride incorporation into a double agar-based solid growth medium

Abstract

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