Expression of matrix metalloproteinase 2 and heat shock protein-72 in immobilized muscle in rats

Eli Carmeli, T. G. Haimovitch, C. E. Nemcovsky

Research output: Contribution to journalArticlepeer-review


Metalloproteinases (MMPs) are proteolytic enzymes that function in the extracellular matrix to degrade connective tissues. While it is clear that certain induced skeletal muscle pathologies promote increased expression of MMP-2 and heat shock protein-72 (HSP-72), the relationship between muscle disuse and expression of MMP-2 and HSP-72 in muscles is unknown. These experiments tested the hypothesis that knee immobilization induced expression of MMP-2 and HSP-72 is disuse-dependent in a way that short-term joint immobilization increases HSV-72 expression, whereas long-term joint immobilization increases MMP-2 expression in skeletal muscles. Male rats (15-months-old) completed 1, 2, 3, and 4 weeks of knee joint immobilization. Muscle mRNA and protein levels of MMP-2 and HSP-72 were assessed in Gastrocnemius (Gast), Superficial and Deep Quadriceps, and Soleus (Sol) muscles by reverse transcriptase-polymerase chain reaction and western blotting, respectively. Results reveal that during the first two weeks of immobilization there is increased protein levels of HSP-72 and expression of mRNA of HSP-72 mainly in slow twitch muscle fibers. However, 3 and 4 weeks of joint immobilization increased both mRNA and protein levels of MMP-2 in skeletal muscles containing a high percentage of fast type II fibers (i.e., Gast and superficial quadriceps). These results support the hypothesis that different periods of muscle disuse induced different proteins expression, and that the influence of joint immobilization on the expression of HSP-72 in the short-term, and MMP-2 in the long ran is associated to fiber types.

Original languageEnglish
Pages (from-to)96-102
Number of pages7
JournalJournal of Musculoskeletal Neuronal Interactions
Issue number1
StatePublished - Jan 2006
Externally publishedYes


  • Atrophy
  • Heat shock protein
  • Immobilization
  • Matrix metalloproteinase
  • Muscle

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Orthopedics and Sports Medicine


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