Evaluation of the NG-Test CARBA 5 Kit for Rapid Detection of Carbapenemase Resistant Enterobacteriaceae

Or Ben-Haim, Maya Azrad, Nora Saleh, Linda Tkhawkho, Avi Peretz

Research output: Contribution to journalArticlepeer-review


Objective: We evaluated NG-Test CARBA 5, a new phenotypic carbapenemase detection assay, and compared it to the routine Xpert CARBA-R polymerase chain reaction assay. Furthermore, we tested the kit's performance after bacterial growth on 4 different solid media. Methods: Seventy carbapenem resistant Enterobacteriaceae (CRE) isolates (60 were carbapenemase producers) were collected at the Poriya Baruch Padeh Medical Center. All isolates were grown on 4 types of agar media - BD BBL CHROMagar carbapenem resistant Enterobacteriaceae, BD CHROMagar Orientation, BD MacConkey II agar, and BD Trypticase Soy Agar II with 5% sheep blood - and were then subjected to NG-Test CARBA 5 kit analysis. Results: The NG-Test CARBA 5 specificity was 100% for all 4 media. However, the sensitivity was higher when bacteria were grown on TSA with 5% sheep blood (98.3%) as compared with the Orientation medium (88.3%), the CPE medium (84.7%), and the MacConkey medium (83.6%). In addition, some of the carbapenemase mechanisms such as Verona Integron-Mediated Metallo-β-lactamase were detected with low agreement levels in specific media but higher agreement levels in the other media. Conclusion: NG-Test CARBA 5 may enable faster detection of carbapenemase producing CRE, which will be of value for treatment adjustment and prevention control. However, the medium type on which the bacteria are grown affects kit sensitivity.

Original languageEnglish
Pages (from-to)375-380
Number of pages6
JournalLaboratory Medicine
Issue number4
StatePublished - 1 Jul 2021
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2020 American Society for Clinical Pathology. All rights reserved.


  • NG-Test Carba 5 kit
  • carbapenem resistant Enterobacteriaceae
  • carbapenemase producing Enterobacteriaceae
  • diagnostic accuracy
  • performance
  • rapid test

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical


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