Ethnopharmacology, phytochemistry and a new chemotaxonomic marker in Oldenlandia affinis (Roem. & Schult.) DC. Rubiaceae

The proper documentation of ethnopharmacological application of widely used indigenous plants and their phytochemical analysis has positively impacted the drug discovery pipeline. Medicinal plants with potential commercial value and prospects for clinical application need to be properly identified and authenticated to avoid confusion, adulteration and substitution. Oldenlandia affinis (OA) has continued to attract scientific attention following the discovery of extremely stable cyclotides (circular peptides) that are not expressed in many investigated members of the contentious genus, Oldenlandia (synonym - Hedyotis); yet there is a lack of an elaborate review covering some broader aspects of its traditional uses, ethnopharmacology and phytochemistry of the species. More importantly, the age long but lingering confusion and taxonomic inconsistencies common to the Oldenlandia-Hedyotis debate could foster species mismatching, increase cases of misidentification, promote adulteration of OA and thereby limit its proper clinical application. Here, we aim to reveal the extent of indigenous use of and research on OA from 1960 till date, unveil knowledge gaps, document hitherto unknown traditional applications, ethnopharmacological uses, pharmacological properties, and reported phytochemical profile. In addition, to encourage proper selection and utilization of genuine crude drug, the chemotaxonomically important phytoconstituents of OA have been presented and the modern approach of chemophenetic study of OA proposed to resolve the lack of consensus in the taxonomy of OA as well as the morphologically and anatomically close members of the taxon. The abundant cyclotide expression in OA represents a new chemotaxonomic marker for its unambiguous identification, utilization and reproducibility of research findings on the species.