Efficient assembly of multi-color fiberless optoelectrodes with on-board light sources for neural stimulation and recording

K. Kampasi, J. Seymour, E. Stark, G. Buzsaki, K. D. Wise, E. Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Fiberless optoelectrodes are an emerging tool to enable brain circuit mapping by providing precise optical modulation and electrical monitoring of many neurons. While optoelectrodes having an on-board light source offer compact and optically efficient device solutions, many of them fail to provide robust thermal and electrical design to fully exploit the recording capabilities of the device. In this work, we present a novel fiberless multicolor optoelectrode solution, which meets the optical and thermal design requirements of an in vivo neural optoelectrode and offers potential for low-noise neural recording. The total optical loss measured for 405 nm and 635 nm wavelengths through the waveguide is 11.7±1.1 dB and 9.9±0.7 dB, corresponding to respective irradiances of 1928 mW/mm2 and 2905 mW/mm2 at the waveguide tip from 6 mW laser diode chips. The efficient thermal packaging enables continuous device operation for up to 190 seconds at 10% duty cycle. We validated the fully packaged device in the intact brain of anesthetized mice co-expressing Channelrhodopsin-2 and Archaerhodopsin in the hippocampal CA1 region and achieved activation and silencing of the same neurons. We discuss improvements made to reduce the stimulation artifact induced by applying currents to the laser diode chips.

Original languageEnglish
Title of host publication2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2016
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages4479-4482
Number of pages4
ISBN (Electronic)9781457702204
DOIs
StatePublished - 13 Oct 2016
Externally publishedYes
Event38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2016 - Orlando, United States
Duration: 16 Aug 201620 Aug 2016

Publication series

NameProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
Volume2016-October
ISSN (Print)1557-170X

Conference

Conference38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2016
Country/TerritoryUnited States
CityOrlando
Period16/08/1620/08/16

Bibliographical note

Publisher Copyright:
© 2016 IEEE.

Keywords

  • ElectroMagnetic Interference
  • Neural Probe
  • Optoelectrodes
  • Optogenetics

ASJC Scopus subject areas

  • Signal Processing
  • Health Informatics
  • Computer Vision and Pattern Recognition
  • Biomedical Engineering

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