Effects of the Hydrogen Sulfide Donor GYY4137 and HSP70 Protein on the Activation of SH-SY5Y Cells by Lipopolysaccharide

M. M. Yurinskaya; D. G. Garbuz; V. N. Afanasiev; M. B. Evgen’ev; M. G. Vinokurov

doi:10.1134/s002689332006014x

Effects of the Hydrogen Sulfide Donor GYY4137 and HSP70 Protein on the Activation of SH-SY5Y Cells by Lipopolysaccharide

M. M. Yurinskaya, D. G. Garbuz, V. N. Afanasiev, M. B. Evgen’ev, M. G. Vinokurov

Research output: Contribution to journal › Article › peer-review

Abstract

Abstract: The effects of exogenous recombinant human heat shock protein Hsp70 and hydrogen sulfide donor GYY4137 on the mechanisms of endocytosis of lipopolysaccharide (LPS) by human neuroblastoma cells SH-SY5Y were studied. Hsp70 and GYY4137 have been shown to significantly reduce LPS-induced production of inflammatory mediators by SH-SY5Y cells, including reactive oxygen species, nitric oxide, TNFα, IL-1β, and IL-6. Both the recombinant protein Hsp70 and the hydrogen sulfide donor GYY4137 exhibited significant protective effects; however, the combined use of these agents did not lead to a cumulative effect. It has been shown that pinocytosis, as well as clathrin-, caveolin-, tubulin- and receptor-dependent endocytosis were involved in protecting the cells by both the hydrogen sulfide donor and Hsp70 from LPS-induced production of reactive oxygen species and NO.

Original language	English
Pages (from-to)	894-903
Number of pages	10
Journal	Molecular Biology
Volume	54
Issue number	6
DOIs	https://doi.org/10.1134/s002689332006014x
State	Published - Nov 2020
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2020, Pleiades Publishing, Inc.

Keywords

GYY4137
Hsp70
NO
SH-SY5Y
endocytosis
lipopolysaccharide
reactive oxygen species

ASJC Scopus subject areas

Biophysics
Structural Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1134/s002689332006014x

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title = "Effects of the Hydrogen Sulfide Donor GYY4137 and HSP70 Protein on the Activation of SH-SY5Y Cells by Lipopolysaccharide",

abstract = "Abstract: The effects of exogenous recombinant human heat shock protein Hsp70 and hydrogen sulfide donor GYY4137 on the mechanisms of endocytosis of lipopolysaccharide (LPS) by human neuroblastoma cells SH-SY5Y were studied. Hsp70 and GYY4137 have been shown to significantly reduce LPS-induced production of inflammatory mediators by SH-SY5Y cells, including reactive oxygen species, nitric oxide, TNFα, IL-1β, and IL-6. Both the recombinant protein Hsp70 and the hydrogen sulfide donor GYY4137 exhibited significant protective effects; however, the combined use of these agents did not lead to a cumulative effect. It has been shown that pinocytosis, as well as clathrin-, caveolin-, tubulin- and receptor-dependent endocytosis were involved in protecting the cells by both the hydrogen sulfide donor and Hsp70 from LPS-induced production of reactive oxygen species and NO.",

keywords = "GYY4137, Hsp70, NO, SH-SY5Y, endocytosis, lipopolysaccharide, reactive oxygen species",

author = "Yurinskaya, {M. M.} and Garbuz, {D. G.} and Afanasiev, {V. N.} and Evgen{\textquoteright}ev, {M. B.} and Vinokurov, {M. G.}",

note = "Publisher Copyright: {\textcopyright} 2020, Pleiades Publishing, Inc.",

year = "2020",

month = nov,

doi = "10.1134/s002689332006014x",

language = "English",

volume = "54",

pages = "894--903",

journal = "Molecular Biology",

issn = "0026-8933",

publisher = "Pleiades Publishing",

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TY - JOUR

T1 - Effects of the Hydrogen Sulfide Donor GYY4137 and HSP70 Protein on the Activation of SH-SY5Y Cells by Lipopolysaccharide

AU - Yurinskaya, M. M.

AU - Garbuz, D. G.

AU - Afanasiev, V. N.

AU - Evgen’ev, M. B.

AU - Vinokurov, M. G.

PY - 2020/11

Y1 - 2020/11

N2 - Abstract: The effects of exogenous recombinant human heat shock protein Hsp70 and hydrogen sulfide donor GYY4137 on the mechanisms of endocytosis of lipopolysaccharide (LPS) by human neuroblastoma cells SH-SY5Y were studied. Hsp70 and GYY4137 have been shown to significantly reduce LPS-induced production of inflammatory mediators by SH-SY5Y cells, including reactive oxygen species, nitric oxide, TNFα, IL-1β, and IL-6. Both the recombinant protein Hsp70 and the hydrogen sulfide donor GYY4137 exhibited significant protective effects; however, the combined use of these agents did not lead to a cumulative effect. It has been shown that pinocytosis, as well as clathrin-, caveolin-, tubulin- and receptor-dependent endocytosis were involved in protecting the cells by both the hydrogen sulfide donor and Hsp70 from LPS-induced production of reactive oxygen species and NO.

AB - Abstract: The effects of exogenous recombinant human heat shock protein Hsp70 and hydrogen sulfide donor GYY4137 on the mechanisms of endocytosis of lipopolysaccharide (LPS) by human neuroblastoma cells SH-SY5Y were studied. Hsp70 and GYY4137 have been shown to significantly reduce LPS-induced production of inflammatory mediators by SH-SY5Y cells, including reactive oxygen species, nitric oxide, TNFα, IL-1β, and IL-6. Both the recombinant protein Hsp70 and the hydrogen sulfide donor GYY4137 exhibited significant protective effects; however, the combined use of these agents did not lead to a cumulative effect. It has been shown that pinocytosis, as well as clathrin-, caveolin-, tubulin- and receptor-dependent endocytosis were involved in protecting the cells by both the hydrogen sulfide donor and Hsp70 from LPS-induced production of reactive oxygen species and NO.

KW - GYY4137

KW - Hsp70

KW - NO

KW - SH-SY5Y

KW - endocytosis

KW - lipopolysaccharide

KW - reactive oxygen species

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U2 - 10.1134/s002689332006014x

DO - 10.1134/s002689332006014x

M3 - Article

AN - SCOPUS:85098730932

SN - 0026-8933

VL - 54

SP - 894

EP - 903

JO - Molecular Biology

JF - Molecular Biology

IS - 6

ER -

Effects of the Hydrogen Sulfide Donor GYY4137 and HSP70 Protein on the Activation of SH-SY5Y Cells by Lipopolysaccharide

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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