Effect of extracellular recombinant human heat shock protein 70 (HSP70) on protein pattern observed after endotoxin-induced macrophage activation

E. A. Rozhkova; O. G. Zatsepina; M. M. Yurinskaya; M. G. Vinokurov; M. B. Evgeniev

doi:10.1134/S0026893311020166

Effect of extracellular recombinant human heat shock protein 70 (HSP70) on protein pattern observed after endotoxin-induced macrophage activation

E. A. Rozhkova, O. G. Zatsepina, M. M. Yurinskaya, M. G. Vinokurov, M. B. Evgeniev

Research output: Contribution to journal › Article › peer-review

Abstract

The protein pattern of murine macrophages cell line J774 has been investigated using 2D electrophoresis after combined action of bacterial endotoxins (LPS), heat shock treatment (HS), and administration of recombinant human Hsp70. The investigation demonstrated significant protective effect of HS and recombinant Hsp70 treatment applied before LPS introduction. This effect is apparently realized by means of several signal transduction systems. In the course of the investigation, we identified eight proteins that exhibited pronounced changes in their synthesis due to combined treatment. The data accumulated may shed light on molecular mechanisms underlying protective antiseptic action of HS and/or recombinant Hsp70 applied before LPS administration.

Original language	English
Pages (from-to)	347-351
Number of pages	5
Journal	Molecular Biology
Volume	45
Issue number	2
DOIs	https://doi.org/10.1134/S0026893311020166
State	Published - Apr 2011
Externally published	Yes

Keywords

2D electrophoresis
bacterial lipopolysaccharides
extracellular heat shock protein 70
macrophages

ASJC Scopus subject areas

Biophysics
Structural Biology

Access to Document

10.1134/S0026893311020166

Cite this

@article{411c77af9b7c4132bc31b818ea99c3ca,

title = "Effect of extracellular recombinant human heat shock protein 70 (HSP70) on protein pattern observed after endotoxin-induced macrophage activation",

abstract = "The protein pattern of murine macrophages cell line J774 has been investigated using 2D electrophoresis after combined action of bacterial endotoxins (LPS), heat shock treatment (HS), and administration of recombinant human Hsp70. The investigation demonstrated significant protective effect of HS and recombinant Hsp70 treatment applied before LPS introduction. This effect is apparently realized by means of several signal transduction systems. In the course of the investigation, we identified eight proteins that exhibited pronounced changes in their synthesis due to combined treatment. The data accumulated may shed light on molecular mechanisms underlying protective antiseptic action of HS and/or recombinant Hsp70 applied before LPS administration.",

keywords = "2D electrophoresis, bacterial lipopolysaccharides, extracellular heat shock protein 70, macrophages",

author = "Rozhkova, {E. A.} and Zatsepina, {O. G.} and Yurinskaya, {M. M.} and Vinokurov, {M. G.} and Evgeniev, {M. B.}",

year = "2011",

month = apr,

doi = "10.1134/S0026893311020166",

language = "English",

volume = "45",

pages = "347--351",

journal = "Molecular Biology",

issn = "0026-8933",

publisher = "Pleiades Publishing",

number = "2",

}

TY - JOUR

T1 - Effect of extracellular recombinant human heat shock protein 70 (HSP70) on protein pattern observed after endotoxin-induced macrophage activation

AU - Rozhkova, E. A.

AU - Zatsepina, O. G.

AU - Yurinskaya, M. M.

AU - Vinokurov, M. G.

AU - Evgeniev, M. B.

PY - 2011/4

Y1 - 2011/4

N2 - The protein pattern of murine macrophages cell line J774 has been investigated using 2D electrophoresis after combined action of bacterial endotoxins (LPS), heat shock treatment (HS), and administration of recombinant human Hsp70. The investigation demonstrated significant protective effect of HS and recombinant Hsp70 treatment applied before LPS introduction. This effect is apparently realized by means of several signal transduction systems. In the course of the investigation, we identified eight proteins that exhibited pronounced changes in their synthesis due to combined treatment. The data accumulated may shed light on molecular mechanisms underlying protective antiseptic action of HS and/or recombinant Hsp70 applied before LPS administration.

AB - The protein pattern of murine macrophages cell line J774 has been investigated using 2D electrophoresis after combined action of bacterial endotoxins (LPS), heat shock treatment (HS), and administration of recombinant human Hsp70. The investigation demonstrated significant protective effect of HS and recombinant Hsp70 treatment applied before LPS introduction. This effect is apparently realized by means of several signal transduction systems. In the course of the investigation, we identified eight proteins that exhibited pronounced changes in their synthesis due to combined treatment. The data accumulated may shed light on molecular mechanisms underlying protective antiseptic action of HS and/or recombinant Hsp70 applied before LPS administration.

KW - 2D electrophoresis

KW - bacterial lipopolysaccharides

KW - extracellular heat shock protein 70

KW - macrophages

UR - http://www.scopus.com/inward/record.url?scp=79955622717&partnerID=8YFLogxK

U2 - 10.1134/S0026893311020166

DO - 10.1134/S0026893311020166

M3 - Article

AN - SCOPUS:79955622717

SN - 0026-8933

VL - 45

SP - 347

EP - 351

JO - Molecular Biology

JF - Molecular Biology

IS - 2

ER -

Effect of extracellular recombinant human heat shock protein 70 (HSP70) on protein pattern observed after endotoxin-induced macrophage activation

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this