Effect of elevated intra-abdominal pressure and 100% oxygen on superior mesenteric artery blood flow and enterocyte turnover in a rat

Igor Sukhotnik; Jorge Mogilner; Lili Hayari; Vera Brod; Ron Shaoul; Nadav Slijper; Y. Bejar; Arnold G. Coran; Haim Bitterman

doi:10.1007/s00383-008-2262-1

Effect of elevated intra-abdominal pressure and 100% oxygen on superior mesenteric artery blood flow and enterocyte turnover in a rat

Igor Sukhotnik, Jorge Mogilner, Lili Hayari, Vera Brod, Ron Shaoul, Nadav Slijper, Y. Bejar, Arnold G. Coran, Haim Bitterman

Research output: Contribution to journal › Article › peer-review

Abstract

Purpose: Elevated intra-abdominal pressure (IAP) has been shown to reduce mesenteric blood flow and cause intestinal damage. The purpose of the present study was to evaluate the effects of IAP and hyperoxia on superior mesenteric artery (SMA) blood flow, enterocyte proliferation and apoptosis in a rat model of abdominal compartment syndrome (ACS). Methods: Male rats underwent midline laparotomy. SMA was isolated and ultrasonic blood flow probes were placed on the vessel for continuous measurement of regional blood flow. Two catheters were introduced into the peritoneal cavity for inflation of air and for measurement of IAP. Rats were divided into three experimental groups: (1) Sham rats were subjected to IAP of 0 mmHg, (2) ACS-air rats were subjected to IAP of 6 mmHg for 2 h and were ventilated with air, and (3) ACS-O₂ rats were subjected to IAP of 6 mmHg and were ventilated with 100% oxygen (O₂) during the operation and for 6 h after the operation. Intestinal structural changes, enterocyte proliferation and enterocyte apoptosis were evaluated at 24 h after operation. A paired Student's t test and the non-parametric Kruskal-Wallis ANOVA test were used as indicated. P < 0.05 was considered statistically significant. Results: IAP at 6 mmHg caused a moderate decrease in SMA blood flow. Inhalation of 100% oxygen resulted in a trend toward an increase in SMA flow when compared to air-ventilated animals. ACS rats demonstrated a significantly lower index of proliferation in jejunum and ileum as well as a significantly greater apoptotic index in jejunum compared to sham animals. Exposure to 100% oxygen resulted in a significant increase in cell proliferation in jejunum and ileum as well as in a significant decrease in cell apoptosis in jejunum compared to air-breathing animals. Conclusions: In a rat model of ACS, elevated IAP decreases SMA blood flow and inhibits enterocyte turnover. Hyperoxia results in a trend toward an increase in SMA blood flow, increases enterocyte proliferation and inhibits cell death via apoptosis. These findings may have significant implications for ventilation strategies during laparoscopy.

Original language	English
Pages (from-to)	1347-1353
Number of pages	7
Journal	Pediatric Surgery International
Volume	24
Issue number	12
DOIs	https://doi.org/10.1007/s00383-008-2262-1
State	Published - Dec 2008
Externally published	Yes

Keywords

Abdominal compartment syndrome
Blood flow
Intestine
Intra-abdominal pressure
Oxygen

ASJC Scopus subject areas

Surgery
Pediatrics, Perinatology, and Child Health

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10.1007/s00383-008-2262-1

Cite this

@article{a9f551f326f946dc8ff018cfa4adadb5,

title = "Effect of elevated intra-abdominal pressure and 100\% oxygen on superior mesenteric artery blood flow and enterocyte turnover in a rat",

abstract = "Purpose: Elevated intra-abdominal pressure (IAP) has been shown to reduce mesenteric blood flow and cause intestinal damage. The purpose of the present study was to evaluate the effects of IAP and hyperoxia on superior mesenteric artery (SMA) blood flow, enterocyte proliferation and apoptosis in a rat model of abdominal compartment syndrome (ACS). Methods: Male rats underwent midline laparotomy. SMA was isolated and ultrasonic blood flow probes were placed on the vessel for continuous measurement of regional blood flow. Two catheters were introduced into the peritoneal cavity for inflation of air and for measurement of IAP. Rats were divided into three experimental groups: (1) Sham rats were subjected to IAP of 0 mmHg, (2) ACS-air rats were subjected to IAP of 6 mmHg for 2 h and were ventilated with air, and (3) ACS-O2 rats were subjected to IAP of 6 mmHg and were ventilated with 100\% oxygen (O2) during the operation and for 6 h after the operation. Intestinal structural changes, enterocyte proliferation and enterocyte apoptosis were evaluated at 24 h after operation. A paired Student's t test and the non-parametric Kruskal-Wallis ANOVA test were used as indicated. P < 0.05 was considered statistically significant. Results: IAP at 6 mmHg caused a moderate decrease in SMA blood flow. Inhalation of 100\% oxygen resulted in a trend toward an increase in SMA flow when compared to air-ventilated animals. ACS rats demonstrated a significantly lower index of proliferation in jejunum and ileum as well as a significantly greater apoptotic index in jejunum compared to sham animals. Exposure to 100\% oxygen resulted in a significant increase in cell proliferation in jejunum and ileum as well as in a significant decrease in cell apoptosis in jejunum compared to air-breathing animals. Conclusions: In a rat model of ACS, elevated IAP decreases SMA blood flow and inhibits enterocyte turnover. Hyperoxia results in a trend toward an increase in SMA blood flow, increases enterocyte proliferation and inhibits cell death via apoptosis. These findings may have significant implications for ventilation strategies during laparoscopy.",

keywords = "Abdominal compartment syndrome, Blood flow, Intestine, Intra-abdominal pressure, Oxygen",

author = "Igor Sukhotnik and Jorge Mogilner and Lili Hayari and Vera Brod and Ron Shaoul and Nadav Slijper and Y. Bejar and Coran, \{Arnold G.\} and Haim Bitterman",

year = "2008",

month = dec,

doi = "10.1007/s00383-008-2262-1",

language = "English",

volume = "24",

pages = "1347--1353",

journal = "Pediatric Surgery International",

issn = "0179-0358",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "12",

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TY - JOUR

T1 - Effect of elevated intra-abdominal pressure and 100% oxygen on superior mesenteric artery blood flow and enterocyte turnover in a rat

AU - Sukhotnik, Igor

AU - Mogilner, Jorge

AU - Hayari, Lili

AU - Brod, Vera

AU - Shaoul, Ron

AU - Slijper, Nadav

AU - Bejar, Y.

AU - Coran, Arnold G.

AU - Bitterman, Haim

PY - 2008/12

Y1 - 2008/12

N2 - Purpose: Elevated intra-abdominal pressure (IAP) has been shown to reduce mesenteric blood flow and cause intestinal damage. The purpose of the present study was to evaluate the effects of IAP and hyperoxia on superior mesenteric artery (SMA) blood flow, enterocyte proliferation and apoptosis in a rat model of abdominal compartment syndrome (ACS). Methods: Male rats underwent midline laparotomy. SMA was isolated and ultrasonic blood flow probes were placed on the vessel for continuous measurement of regional blood flow. Two catheters were introduced into the peritoneal cavity for inflation of air and for measurement of IAP. Rats were divided into three experimental groups: (1) Sham rats were subjected to IAP of 0 mmHg, (2) ACS-air rats were subjected to IAP of 6 mmHg for 2 h and were ventilated with air, and (3) ACS-O2 rats were subjected to IAP of 6 mmHg and were ventilated with 100% oxygen (O2) during the operation and for 6 h after the operation. Intestinal structural changes, enterocyte proliferation and enterocyte apoptosis were evaluated at 24 h after operation. A paired Student's t test and the non-parametric Kruskal-Wallis ANOVA test were used as indicated. P < 0.05 was considered statistically significant. Results: IAP at 6 mmHg caused a moderate decrease in SMA blood flow. Inhalation of 100% oxygen resulted in a trend toward an increase in SMA flow when compared to air-ventilated animals. ACS rats demonstrated a significantly lower index of proliferation in jejunum and ileum as well as a significantly greater apoptotic index in jejunum compared to sham animals. Exposure to 100% oxygen resulted in a significant increase in cell proliferation in jejunum and ileum as well as in a significant decrease in cell apoptosis in jejunum compared to air-breathing animals. Conclusions: In a rat model of ACS, elevated IAP decreases SMA blood flow and inhibits enterocyte turnover. Hyperoxia results in a trend toward an increase in SMA blood flow, increases enterocyte proliferation and inhibits cell death via apoptosis. These findings may have significant implications for ventilation strategies during laparoscopy.

AB - Purpose: Elevated intra-abdominal pressure (IAP) has been shown to reduce mesenteric blood flow and cause intestinal damage. The purpose of the present study was to evaluate the effects of IAP and hyperoxia on superior mesenteric artery (SMA) blood flow, enterocyte proliferation and apoptosis in a rat model of abdominal compartment syndrome (ACS). Methods: Male rats underwent midline laparotomy. SMA was isolated and ultrasonic blood flow probes were placed on the vessel for continuous measurement of regional blood flow. Two catheters were introduced into the peritoneal cavity for inflation of air and for measurement of IAP. Rats were divided into three experimental groups: (1) Sham rats were subjected to IAP of 0 mmHg, (2) ACS-air rats were subjected to IAP of 6 mmHg for 2 h and were ventilated with air, and (3) ACS-O2 rats were subjected to IAP of 6 mmHg and were ventilated with 100% oxygen (O2) during the operation and for 6 h after the operation. Intestinal structural changes, enterocyte proliferation and enterocyte apoptosis were evaluated at 24 h after operation. A paired Student's t test and the non-parametric Kruskal-Wallis ANOVA test were used as indicated. P < 0.05 was considered statistically significant. Results: IAP at 6 mmHg caused a moderate decrease in SMA blood flow. Inhalation of 100% oxygen resulted in a trend toward an increase in SMA flow when compared to air-ventilated animals. ACS rats demonstrated a significantly lower index of proliferation in jejunum and ileum as well as a significantly greater apoptotic index in jejunum compared to sham animals. Exposure to 100% oxygen resulted in a significant increase in cell proliferation in jejunum and ileum as well as in a significant decrease in cell apoptosis in jejunum compared to air-breathing animals. Conclusions: In a rat model of ACS, elevated IAP decreases SMA blood flow and inhibits enterocyte turnover. Hyperoxia results in a trend toward an increase in SMA blood flow, increases enterocyte proliferation and inhibits cell death via apoptosis. These findings may have significant implications for ventilation strategies during laparoscopy.

KW - Abdominal compartment syndrome

KW - Blood flow

KW - Intestine

KW - Intra-abdominal pressure

KW - Oxygen

UR - https://www.scopus.com/pages/publications/56649099049

U2 - 10.1007/s00383-008-2262-1

DO - 10.1007/s00383-008-2262-1

M3 - Article

C2 - 18956202

AN - SCOPUS:56649099049

SN - 0179-0358

VL - 24

SP - 1347

EP - 1353

JO - Pediatric Surgery International

JF - Pediatric Surgery International

IS - 12

ER -

Effect of elevated intra-abdominal pressure and 100% oxygen on superior mesenteric artery blood flow and enterocyte turnover in a rat

Abstract

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