Dynamic macromolecular composition and high exudation rates in Prochlorococcus

Dalit Roth-Rosenberg, Dikla Aharonovich, Anne Willem Omta, Michael J. Follows, Daniel Sher

Research output: Contribution to journalArticlepeer-review

Abstract

Every living cell is composed of macromolecules such as proteins, DNA, RNA, and pigments. The ratio between these macromolecular pools depends on the allocation of resources within the organism to different physiological requirements, and in turn affects biogeochemical cycles of elements such as carbon, nitrogen, and phosphorus. Here, we present detailed measurements of the macromolecular composition of Prochlorococcus MIT9312, a representative strain of a globally abundant marine primary producer, as it grows and declines due to nitrogen starvation in laboratory batch cultures. As cells reached stationary stage and declined, protein per cell decreased by ~ 30% whereas RNA per cell and pigments per cell decreased by ~ 75%. The decline stage was associated with the appearance of chlorotic cells, which had higher forward scatter (a proxy for cell size) but lower chlorophyll autofluorescence, as well as with changes in photosynthetic pigment composition. Specifically, during culture decline divinyl-chlorophyll-like pigments emerged, which were not observed during exponential growth. These divinyl-chlorophyll-like pigments were also observed in natural samples from the Eastern Mediterranean. Over 90% of the carbon fixed by Prochlorococcus MIT9312 (but not of a different strain, NATL2A) was released into the growth media under these laboratory conditions. Variations in RNA/protein indicate that, broadly defined, the macromolecular composition of Prochlorococcus MIT9312 is more similar to eukaryotic phytoplankton than to marine heterotrophic bacteria, possibly due to the significant investment in photosynthetic machinery of phototrophs.

Original languageEnglish
Pages (from-to)1759-1773
Number of pages15
JournalLimnology and Oceanography
Volume66
Issue number5
DOIs
StatePublished - May 2021

Bibliographical note

Funding Information:
We thank Hila Elifantz, Ilana Berman‐Frank, Eyal Geisler and Edo Bar‐Zeev for the TOC analyses and Jack Silberman for the DIC analysis. This study was supported by the Gordon & Betty Moore Foundation (grant number GBMF #3778, to MJF), by the Human Frontiers Science Program (grant number grant RGP0020/2016, to DS) and by the United States‐Israel Binational Science Foundation (Grant number 2010183 to DS and MJF and grant number 2016532 to DS).

Publisher Copyright:
© 2021 Association for the Sciences of Limnology and Oceanography.

ASJC Scopus subject areas

  • Oceanography
  • Aquatic Science

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