Drosophila model for studying phagocytosis following neuronal cell death

Boris Shklyar, Flonia Levy-Adam, Estee Kurant

Research output: Contribution to journalArticlepeer-review

Abstract

During central nervous system (CNS) development, a large number of neurons die by apoptosis and are efficiently removed through phagocytosis. Since apoptosis and apoptotic cell clearance are highly conserved in evolution, relatively simple and easily accessible Drosophila embryonic CNS provides a good model to study molecular and cellular mechanisms of these processes. Here, we describe how to assess neuronal apoptosis and glial phagocytosis of apoptotic neurons using immunohistochemistry of whole fixed embryos and live imaging of developing embryonic CNS. Combination of these different strategies allows a comprehensive analysis of neuronal cell death in vivo.

Original languageEnglish
Pages (from-to)359-368
Number of pages10
JournalMethods in Molecular Biology
Volume1254
DOIs
StatePublished - 2015

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015.

Keywords

  • Apoptosis
  • CNS
  • Drosophila
  • Embryo
  • Phagocytosis

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

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