Drosophila GATA factor serpent establishes phagocytic ability of embryonic macrophages

Evgeny Shlyakhover, Boris Shklyar, Ketty Hakim-Mishnaevski, Flonia Levy-Adam, Estee Kurant

Research output: Contribution to journalArticlepeer-review


During Drosophila embryogenesis, a large number of apoptotic cells are efficiently engulfed and degraded by professional phagocytes, macrophages. Phagocytic receptors Six-Microns-Under (SIMU), Draper (Drpr) and Croquemort (Crq) are specifically expressed in embryonic macrophages and required for their phagocytic function. However, how this function is established during development remains unclear. Here we demonstrate that the key regulator of Drosophila embryonic hemocyte differentiation, the transcription factor Serpent (Srp), plays a central role in establishing macrophage phagocytic competence. Srp, a homolog of the mammalian GATA factors, is required and sufficient for the specific expression of SIMU, Drpr and Crq receptors in embryonic macrophages. Moreover, we show that each of these receptors can significantly rescue phagocytosis defects of macrophages in srp mutants, including their distribution in the embryo and engulfment of apoptotic cells. This reveals that the proficiency of macrophages to remove apoptotic cells relies on the expression of SIMU, Crq and/or Drpr. However, Glial Cells Missing (GCM) acting downstream of Srp in the differentiation of hemocytes, is dispensable for their phagocytic function during embryogenesis. Taken together, our study discloses the molecular mechanism underlying the development of macrophages as skilled phagocytes of apoptotic cells.

Original languageEnglish
Article number266
JournalFrontiers in Immunology
Issue numberMAR
StatePublished - 8 Mar 2018

Bibliographical note

Publisher Copyright:
© 2018 Shlyakhover, Shklyar, Hakim-Mishnaevski, Levy-Adam and Kurant.


  • Apoptosis
  • Development
  • Drosophila
  • GATA
  • Macrophages
  • Phagocytosis
  • SIMU
  • Serpent

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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