Draft genome of Rosenbergiella nectarea strain 8N4T provides insights into the potential role of this species in its plant host

Sivan Laviad-Shitrit; Ido Izhaki; William B. Whitman; Nicole Shapiro; Tanja Woyke; Nikos C. Kyrpides; Malka Halpern

doi:10.7717/peerj.8822

Draft genome of Rosenbergiella nectarea strain 8N4^T provides insights into the potential role of this species in its plant host

Sivan Laviad-Shitrit, Ido Izhaki, William B. Whitman, Nicole Shapiro, Tanja Woyke, Nikos C. Kyrpides, Malka Halpern

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Rosenbergiella nectarea strain 8N4T, the type species of the genus Rosenbergiella, was isolated from Amygdalus communis (almond) floral nectar. Other strains of this species were isolated from the floral nectar of Citrus paradisi (grapefruit), Nicotiana glauca (tobacco tree) and from Asphodelus aestivus. R. nectarea strain 8N4^T is a Gram-negative, oxidase-negative, facultatively anaerobic bacterium in the family Enterobacteriaceae. Results: Here we describe features of this organism, together with its genome sequence and annotation. The DNA GC content is 47.38%, the assembly size is 3,294,717 bp, and the total number of genes are 3,346. The genome discloses the possible role that this species may play in the plant. The genome contains both virulence genes, like pectin lyase and hemolysin, that may harm plant cells and genes that are predicted to produce volatile compounds that may impact the visitation rates by nectar consumers, such as pollinators and nectar thieves. Conclusions: The genome of R. nectarea strain 8N4^T reveals a mutualistic interaction with the plant host and a possible effect on plant pollination and fitness.

Original language	English
Article number	8822
Journal	PeerJ
Volume	2020
Issue number	4
DOIs	https://doi.org/10.7717/peerj.8822
State	Published - 2020

Bibliographical note

Funding Information:
This project has been supported by the Community Sequencing Program of the U.S. Department of Energy's Joint Genome Institute. The sequencing, assembly and automated genome analysis work at the DOE-JGI was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. This work was also supported by a grant from the Israel Science Foundation (ISF, grant no. 296/16; Prof. Ido Izhaki and Prof. Malka Halpern, PIs). There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Funding Information:
This project has been supported by the Community Sequencing Program of the U.S. Department of Energy’s Joint Genome Institute. The sequencing, assembly and automated genome analysis work at the DOE-JGI was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. This work was also supported by a grant from the Israel Science Foundation (ISF, grant no. 296/16; Prof. Ido Izhaki and Prof. Malka Halpern, PIs). There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Publisher Copyright:
© 2020 Journal of Global Pharma Technology. All rights reserved.

Keywords

Attractant
Flower
Nectar
Pectinase
Pyocin
Repellant
Rosenbergiella nectarea
Whole genome

ASJC Scopus subject areas

Neuroscience (all)
Biochemistry, Genetics and Molecular Biology (all)
Agricultural and Biological Sciences (all)

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10.7717/peerj.8822

Cite this

@article{94bf564174f4437497f5ba4918ff6677,

title = "Draft genome of Rosenbergiella nectarea strain 8N4T provides insights into the potential role of this species in its plant host",

abstract = "Background: Rosenbergiella nectarea strain 8N4T, the type species of the genus Rosenbergiella, was isolated from Amygdalus communis (almond) floral nectar. Other strains of this species were isolated from the floral nectar of Citrus paradisi (grapefruit), Nicotiana glauca (tobacco tree) and from Asphodelus aestivus. R. nectarea strain 8N4T is a Gram-negative, oxidase-negative, facultatively anaerobic bacterium in the family Enterobacteriaceae. Results: Here we describe features of this organism, together with its genome sequence and annotation. The DNA GC content is 47.38%, the assembly size is 3,294,717 bp, and the total number of genes are 3,346. The genome discloses the possible role that this species may play in the plant. The genome contains both virulence genes, like pectin lyase and hemolysin, that may harm plant cells and genes that are predicted to produce volatile compounds that may impact the visitation rates by nectar consumers, such as pollinators and nectar thieves. Conclusions: The genome of R. nectarea strain 8N4T reveals a mutualistic interaction with the plant host and a possible effect on plant pollination and fitness.",

keywords = "Attractant, Flower, Nectar, Pectinase, Pyocin, Repellant, Rosenbergiella nectarea, Whole genome",

author = "Sivan Laviad-Shitrit and Ido Izhaki and Whitman, {William B.} and Nicole Shapiro and Tanja Woyke and Kyrpides, {Nikos C.} and Malka Halpern",

note = "Funding Information: This project has been supported by the Community Sequencing Program of the U.S. Department of Energy's Joint Genome Institute. The sequencing, assembly and automated genome analysis work at the DOE-JGI was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. This work was also supported by a grant from the Israel Science Foundation (ISF, grant no. 296/16; Prof. Ido Izhaki and Prof. Malka Halpern, PIs). There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Funding Information: This project has been supported by the Community Sequencing Program of the U.S. Department of Energy{\textquoteright}s Joint Genome Institute. The sequencing, assembly and automated genome analysis work at the DOE-JGI was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. This work was also supported by a grant from the Israel Science Foundation (ISF, grant no. 296/16; Prof. Ido Izhaki and Prof. Malka Halpern, PIs). There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: {\textcopyright} 2020 Journal of Global Pharma Technology. All rights reserved.",

year = "2020",

doi = "10.7717/peerj.8822",

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volume = "2020",

journal = "PeerJ",

issn = "2167-8359",

publisher = "PeerJ Inc.",

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TY - JOUR

T1 - Draft genome of Rosenbergiella nectarea strain 8N4T provides insights into the potential role of this species in its plant host

AU - Laviad-Shitrit, Sivan

AU - Izhaki, Ido

AU - Whitman, William B.

AU - Shapiro, Nicole

AU - Woyke, Tanja

AU - Kyrpides, Nikos C.

AU - Halpern, Malka

N1 - Funding Information: This project has been supported by the Community Sequencing Program of the U.S. Department of Energy's Joint Genome Institute. The sequencing, assembly and automated genome analysis work at the DOE-JGI was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. This work was also supported by a grant from the Israel Science Foundation (ISF, grant no. 296/16; Prof. Ido Izhaki and Prof. Malka Halpern, PIs). There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Funding Information: This project has been supported by the Community Sequencing Program of the U.S. Department of Energy’s Joint Genome Institute. The sequencing, assembly and automated genome analysis work at the DOE-JGI was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. This work was also supported by a grant from the Israel Science Foundation (ISF, grant no. 296/16; Prof. Ido Izhaki and Prof. Malka Halpern, PIs). There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: © 2020 Journal of Global Pharma Technology. All rights reserved.

PY - 2020

Y1 - 2020

N2 - Background: Rosenbergiella nectarea strain 8N4T, the type species of the genus Rosenbergiella, was isolated from Amygdalus communis (almond) floral nectar. Other strains of this species were isolated from the floral nectar of Citrus paradisi (grapefruit), Nicotiana glauca (tobacco tree) and from Asphodelus aestivus. R. nectarea strain 8N4T is a Gram-negative, oxidase-negative, facultatively anaerobic bacterium in the family Enterobacteriaceae. Results: Here we describe features of this organism, together with its genome sequence and annotation. The DNA GC content is 47.38%, the assembly size is 3,294,717 bp, and the total number of genes are 3,346. The genome discloses the possible role that this species may play in the plant. The genome contains both virulence genes, like pectin lyase and hemolysin, that may harm plant cells and genes that are predicted to produce volatile compounds that may impact the visitation rates by nectar consumers, such as pollinators and nectar thieves. Conclusions: The genome of R. nectarea strain 8N4T reveals a mutualistic interaction with the plant host and a possible effect on plant pollination and fitness.

AB - Background: Rosenbergiella nectarea strain 8N4T, the type species of the genus Rosenbergiella, was isolated from Amygdalus communis (almond) floral nectar. Other strains of this species were isolated from the floral nectar of Citrus paradisi (grapefruit), Nicotiana glauca (tobacco tree) and from Asphodelus aestivus. R. nectarea strain 8N4T is a Gram-negative, oxidase-negative, facultatively anaerobic bacterium in the family Enterobacteriaceae. Results: Here we describe features of this organism, together with its genome sequence and annotation. The DNA GC content is 47.38%, the assembly size is 3,294,717 bp, and the total number of genes are 3,346. The genome discloses the possible role that this species may play in the plant. The genome contains both virulence genes, like pectin lyase and hemolysin, that may harm plant cells and genes that are predicted to produce volatile compounds that may impact the visitation rates by nectar consumers, such as pollinators and nectar thieves. Conclusions: The genome of R. nectarea strain 8N4T reveals a mutualistic interaction with the plant host and a possible effect on plant pollination and fitness.

KW - Attractant

KW - Flower

KW - Nectar

KW - Pectinase

KW - Pyocin

KW - Repellant

KW - Rosenbergiella nectarea

KW - Whole genome

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DO - 10.7717/peerj.8822

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