Divergent evolutionary histories of DNA markers in a Hawaiian population of the coral Montipora capitata

Hollie M. Putnam; Diane K. Adams; Ehud Zelzion; Nicole E. Wagner; Huan Qiu; Tali Mass; Paul G. Falkowski; Ruth D. Gates; Debashish Bhattacharya

doi:10.7717/peerj.3319

Divergent evolutionary histories of DNA markers in a Hawaiian population of the coral Montipora capitata

Hollie M. Putnam, Diane K. Adams, Ehud Zelzion, Nicole E. Wagner, Huan Qiu, Tali Mass, Paul G. Falkowski, Ruth D. Gates, Debashish Bhattacharya

Department of Marine Biology

Research output: Contribution to journal › Article › peer-review

Abstract

We investigated intra- and inter-colony sequence variation in a population of the dom-inant Hawaiian coral Montipora capitata by analyzing marker gene and genomic data. Ribosomal ITS1 regions showed evidence of a reticulate history among the colonies, suggesting incomplete rDNA repeat homogenization. Analysis of the mitochondrial genome identified a major (M. capitata) and a minor (M. flabellata) haplotype in single polyp-derived sperm bundle DNA with some colonies containing 2-3 different mtDNA haplotypes. In contrast, Pax-C and newly identified single-copy nuclear genes showed either no sequence differences or minor variations in SNP frequencies segregating among the colonies. Our data suggest past mitochondrial introgression in M. capitata, whereas nuclear single-copy loci show limited variation, highlighting the divergent evolutionary histories of these coral DNA markers.

Original language	English
Article number	e3319
Journal	PeerJ
Volume	2017
Issue number	5
DOIs	https://doi.org/10.7717/peerj.3319
State	Published - 2017

Bibliographical note

Publisher Copyright:
© 2017 Putnam et al.

Keywords

Coral colonies
Genomics
Sperm bundle DNA

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences

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10.7717/peerj.3319

Cite this

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title = "Divergent evolutionary histories of DNA markers in a Hawaiian population of the coral Montipora capitata",

abstract = "We investigated intra- and inter-colony sequence variation in a population of the dom-inant Hawaiian coral Montipora capitata by analyzing marker gene and genomic data. Ribosomal ITS1 regions showed evidence of a reticulate history among the colonies, suggesting incomplete rDNA repeat homogenization. Analysis of the mitochondrial genome identified a major (M. capitata) and a minor (M. flabellata) haplotype in single polyp-derived sperm bundle DNA with some colonies containing 2-3 different mtDNA haplotypes. In contrast, Pax-C and newly identified single-copy nuclear genes showed either no sequence differences or minor variations in SNP frequencies segregating among the colonies. Our data suggest past mitochondrial introgression in M. capitata, whereas nuclear single-copy loci show limited variation, highlighting the divergent evolutionary histories of these coral DNA markers.",

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AU - Adams, Diane K.

AU - Zelzion, Ehud

AU - Wagner, Nicole E.

AU - Qiu, Huan

AU - Mass, Tali

AU - Falkowski, Paul G.

AU - Gates, Ruth D.

AU - Bhattacharya, Debashish

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KW - Sperm bundle DNA

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Divergent evolutionary histories of DNA markers in a Hawaiian population of the coral Montipora capitata

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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