We investigated intra- and inter-colony sequence variation in a population of the dom-inant Hawaiian coral Montipora capitata by analyzing marker gene and genomic data. Ribosomal ITS1 regions showed evidence of a reticulate history among the colonies, suggesting incomplete rDNA repeat homogenization. Analysis of the mitochondrial genome identified a major (M. capitata) and a minor (M. flabellata) haplotype in single polyp-derived sperm bundle DNA with some colonies containing 2-3 different mtDNA haplotypes. In contrast, Pax-C and newly identified single-copy nuclear genes showed either no sequence differences or minor variations in SNP frequencies segregating among the colonies. Our data suggest past mitochondrial introgression in M. capitata, whereas nuclear single-copy loci show limited variation, highlighting the divergent evolutionary histories of these coral DNA markers.
Bibliographical noteFunding Information:
This work was made possible by grants from the National Science Foundation EF-1416785 awarded to P.G.F., T.M. and D.B, OCE-PRF 1323822 to H.M.P., and Hawaii EPSCoR EPS-0903833. Funding was also provided by the Paul G. Allen Family Foundation to R.D.G. and by the US-Israel Binational Science Foundation (BSF-2014035) to D.K.A. and T.M. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
© 2017 Putnam et al.
- Coral colonies
- Sperm bundle DNA
ASJC Scopus subject areas
- Neuroscience (all)
- Biochemistry, Genetics and Molecular Biology (all)
- Agricultural and Biological Sciences (all)