Dissociation of circadian and circatidal timekeeping in the marine crustacean eurydice pulchra

Lin Zhang, Michael H. Hastings, Edward W. Green, Eran Tauber, Martin Sladek, Simon G. Webster, Charalambos P. Kyriacou, David C. Wilcockson

Research output: Contribution to journalArticlepeer-review

Abstract

Background Tidal (12.4 hr) cycles of behavior and physiology adapt intertidal organisms to temporally complex coastal environments, yet their underlying mechanism is unknown. However, the very existence of an independent "circatidal" clock has been disputed, and it has been argued that tidal rhythms arise as a submultiple of a circadian clock, operating in dual oscillators whose outputs are held in antiphase i.e., ∼12.4 hr apart. Results We demonstrate that the intertidal crustacean Eurydice pulchra (Leach) exhibits robust tidal cycles of swimming in parallel to circadian (24 hr) rhythms in behavioral, physiological and molecular phenotypes. Importantly, ∼12.4 hr cycles of swimming are sustained in constant conditions, they can be entrained by suitable stimuli, and they are temperature compensated, thereby meeting the three criteria that define a biological clock. Unexpectedly, tidal rhythms (like circadian rhythms) are sensitive to pharmacological inhibition of Casein kinase 1, suggesting the possibility of shared clock substrates. However, cloning the canonical circadian genes of E. pulchra to provide molecular markers of circadian timing and also reagents to disrupt it by RNAi revealed that environmental and molecular manipulations that confound circadian timing do not affect tidal timing. Thus, competent circadian timing is neither an inevitable nor necessary element of tidal timekeeping. Conclusions We demonstrate that tidal rhythms are driven by a dedicated circatidal pacemaker that is distinct from the circadian system of E. pulchra, thereby resolving a long-standing debate regarding the nature of the circatidal mechanism.

Original languageEnglish
Pages (from-to)1863-1873
Number of pages11
JournalCurrent Biology
Volume23
Issue number19
DOIs
StatePublished - 7 Oct 2013
Externally publishedYes

Bibliographical note

Funding Information:
C.P.K. and S.G.W. thank the BBSRC for funding (91/517172, BBE0017501, BB/E000835/1, and BB/K009702/1) and the College of Medicine and Biology at the University of Leicester for funding L.Z. for 1 year. M.H.H. was supported by the Medical Research Council, UK. We thank Horacio de la Iglesias for sharing unpublished sequencing information.

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (all)
  • Agricultural and Biological Sciences (all)

Fingerprint

Dive into the research topics of 'Dissociation of circadian and circatidal timekeeping in the marine crustacean eurydice pulchra'. Together they form a unique fingerprint.

Cite this