TY - JOUR
T1 - Dissemination and characterization of Escherichia coli resistant to extended-cephalosporins in feedlot lambs
T2 - A two-year two-population study
AU - Gozi, Katia Suemi
AU - da Silva, Caroline Rodrigues
AU - do Valle Barroso, Marlon
AU - Barboza, Jessica Priscila
AU - Peiró, Juliana Regina
AU - Madec, Jean Yves
AU - Haenni, Marisa
AU - Mendes, Luiz Claudio Nogueira
AU - Nogueira, Mara Corrêa Lelles
AU - Casella, Tiago
N1 - Publisher Copyright:
© 2024 Elsevier Ltd
PY - 2024/11
Y1 - 2024/11
N2 - Dissemination of antimicrobial resistance in food animals is a One Health concern, but sheep production has been overlooked. This study aimed to explore the dissemination of Escherichia coli resistant to extended-spectrum cephalosporins (ESC) in feedlot lambs. Two pens were sampled on two occasions, and carcasses and other mammals living around were also tested. E. coli were recovered and antibiotic resistance determined. blaCTX-M/CMY genes and their genetic localization were characterized. Whole genome sequencing (WGS) was performed to confirm clonal relationship. The most prevalent ESC-resistance genes in the 108 E. coli isolates were blaCTX-M-55 (53.7 %), blaCTX-M-2 (14.8 %) and blaCMY-2 (13.9 %). Most blaCTX-M-55 genes were found on the chromosome, but IncA/C, IncHI1, IncHI2 and IncF plasmids were also identified. Genetic diversity was observed even though ST6448 was by far the most frequent ST. WGS analysis showed high similarity among isolates recovered from feedlot lambs, animals in the surroundings and lambs’ carcasses, proving the clonal and plasmidic dissemination.
AB - Dissemination of antimicrobial resistance in food animals is a One Health concern, but sheep production has been overlooked. This study aimed to explore the dissemination of Escherichia coli resistant to extended-spectrum cephalosporins (ESC) in feedlot lambs. Two pens were sampled on two occasions, and carcasses and other mammals living around were also tested. E. coli were recovered and antibiotic resistance determined. blaCTX-M/CMY genes and their genetic localization were characterized. Whole genome sequencing (WGS) was performed to confirm clonal relationship. The most prevalent ESC-resistance genes in the 108 E. coli isolates were blaCTX-M-55 (53.7 %), blaCTX-M-2 (14.8 %) and blaCMY-2 (13.9 %). Most blaCTX-M-55 genes were found on the chromosome, but IncA/C, IncHI1, IncHI2 and IncF plasmids were also identified. Genetic diversity was observed even though ST6448 was by far the most frequent ST. WGS analysis showed high similarity among isolates recovered from feedlot lambs, animals in the surroundings and lambs’ carcasses, proving the clonal and plasmidic dissemination.
KW - Beta-lactam resistance
KW - One Health
KW - Phylogeny
KW - Sheep
UR - http://www.scopus.com/inward/record.url?scp=85206235837&partnerID=8YFLogxK
U2 - 10.1016/j.cimid.2024.102258
DO - 10.1016/j.cimid.2024.102258
M3 - Article
C2 - 39413622
AN - SCOPUS:85206235837
SN - 0147-9571
VL - 114
JO - Comparative Immunology, Microbiology and Infectious Diseases
JF - Comparative Immunology, Microbiology and Infectious Diseases
M1 - 102258
ER -