Abstract
The accessibility and transparency of the cornea permit robust stem cell labeling and in vivo cell fate mapping. Limbal epithelial stem cells (LSCs) that renew the cornea are traditionally viewed as rare, slow-cycling cells that follow deterministic rules dictating their self-renewal or differentiation. Here, we combined single-cell RNA sequencing and advanced quantitative lineage tracing for in-depth analysis of the murine limbal epithelium. These analysis revealed the co-existence of two LSC populations localized in separate and well-defined sub-compartments, termed the “outer” and “inner” limbus. The primitive population of quiescent outer LSCs participates in wound healing and boundary formation, and these cells are regulated by T cells, which serve as a niche. In contrast, the inner peri-corneal limbus hosts active LSCs that maintain corneal epithelial homeostasis. Quantitative analyses suggest that LSC populations are abundant, following stochastic rules and neutral drift dynamics. Together these results demonstrate that discrete LSC populations mediate corneal homeostasis and regeneration.
Original language | English |
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Pages (from-to) | 1248-1261.e8 |
Journal | Cell Stem Cell |
Volume | 28 |
Issue number | 7 |
DOIs | |
State | Published - 1 Jul 2021 |
Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2021 Elsevier Inc.
Keywords
- cornea
- immune cells
- limbal stem cells
- limbus
- lineage tracing
- mathematical modeling
- niche
- single cell RNA sequencing
- stem cell dynamics
ASJC Scopus subject areas
- Molecular Medicine
- Genetics
- Cell Biology