Discovery of stripe rust resistance with incomplete dominance in wild emmer wheat using bulked segregant analysis sequencing

Valentyna Klymiuk; Harmeet Singh Chawla; Krystalee Wiebe; Jennifer Ens; Andrii Fatiukha; Liubov Govta; Tzion Fahima; Curtis J. Pozniak

doi:10.1038/s42003-022-03773-3

Discovery of stripe rust resistance with incomplete dominance in wild emmer wheat using bulked segregant analysis sequencing

Valentyna Klymiuk, Harmeet Singh Chawla, Krystalee Wiebe, Jennifer Ens, Andrii Fatiukha, Liubov Govta, Tzion Fahima, Curtis J. Pozniak

Department of Evolutionary and Environmental Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Durable crop disease resistance is an essential component of global food security. Continuous pathogen evolution leads to a breakdown of resistance and there is a pressing need to characterize new resistance genes for use in plant breeding. Here we identified an accession of wild emmer wheat (Triticum turgidum ssp. dicoccoides), PI 487260, that is highly resistant to multiple stripe rust isolates. Genetic analysis revealed resistance was conferred by a single, incompletely dominant gene designated as Yr84. Through bulked segregant analysis sequencing (BSA-Seq) we identified a 52.7 Mb resistance-associated interval on chromosome 1BS. Detected variants were used to design genetic markers for recombinant screening, further refining the interval of Yr84 to a 2.3–3.3 Mb in tetraploid wheat genomes. This interval contains 34 candidate genes encoding for protein domains involved in disease resistance responses. Furthermore, KASP markers closely-linked to Yr84 were developed to facilitate marker-assisted selection for rust resistance breeding.

Original language	English
Article number	826
Pages (from-to)	826
Journal	Communications Biology
Volume	5
Issue number	1
DOIs	https://doi.org/10.1038/s42003-022-03773-3
State	Published - 17 Aug 2022

Bibliographical note

Funding Information:
The authors are thankful to Dr. Xianming Chen (USDA) and Dr. Randy Kutcher (University of Saskatchewan) for making stripe rust races available, and to USDA-ARS National Small Grains Collection and Dr. Peng Zhang (University of Sydney) for providing seed material. The authors are grateful to Madison Kist, Xue (Snow) Lin, Lexie Gerl, Ayla Lichtenwald and Maureen Troesch for their technical support. This work was conducted through the 4D Wheat Project (C.J.P.) supported by Genome Canada, Genome Prairie, the Saskatchewan Ministry of Agriculture, Saskatchewan Wheat Development Commission, Alberta Wheat Development Commission, Secan, Agriculture and Agri-Food Canada and the Manitoba Crop Alliance; the “Maximizing Durable Disease Resistance in Wheat” Project (C.J.P.) sponsored by the Saskatchewan Ministry of Agriculture grant number ADF20200353; the Israel Science Foundation, grant number 1366/18 (T.F.).

Funding Information:
The authors are thankful to Dr. Xianming Chen (USDA) and Dr. Randy Kutcher (University of Saskatchewan) for making stripe rust races available, and to USDA-ARS National Small Grains Collection and Dr. Peng Zhang (University of Sydney) for providing seed material. The authors are grateful to Madison Kist, Xue (Snow) Lin, Lexie Gerl, Ayla Lichtenwald and Maureen Troesch for their technical support. This work was conducted through the 4D Wheat Project (C.J.P.) supported by Genome Canada, Genome Prairie, the Saskatchewan Ministry of Agriculture, Saskatchewan Wheat Development Commission, Alberta Wheat Development Commission, Secan, Agriculture and Agri-Food Canada and the Manitoba Crop Alliance; the “Maximizing Durable Disease Resistance in Wheat” Project (C.J.P.) sponsored by the Saskatchewan Ministry of Agriculture grant number ADF20200353; the Israel Science Foundation, grant number 1366/18 (T.F.).

Publisher Copyright:
© 2022, The Author(s).

ASJC Scopus subject areas

Medicine (miscellaneous)
Biochemistry, Genetics and Molecular Biology (all)
Agricultural and Biological Sciences (all)

Access to Document

10.1038/s42003-022-03773-3

Cite this

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title = "Discovery of stripe rust resistance with incomplete dominance in wild emmer wheat using bulked segregant analysis sequencing",

abstract = "Durable crop disease resistance is an essential component of global food security. Continuous pathogen evolution leads to a breakdown of resistance and there is a pressing need to characterize new resistance genes for use in plant breeding. Here we identified an accession of wild emmer wheat (Triticum turgidum ssp. dicoccoides), PI 487260, that is highly resistant to multiple stripe rust isolates. Genetic analysis revealed resistance was conferred by a single, incompletely dominant gene designated as Yr84. Through bulked segregant analysis sequencing (BSA-Seq) we identified a 52.7 Mb resistance-associated interval on chromosome 1BS. Detected variants were used to design genetic markers for recombinant screening, further refining the interval of Yr84 to a 2.3–3.3 Mb in tetraploid wheat genomes. This interval contains 34 candidate genes encoding for protein domains involved in disease resistance responses. Furthermore, KASP markers closely-linked to Yr84 were developed to facilitate marker-assisted selection for rust resistance breeding.",

author = "Valentyna Klymiuk and Chawla, {Harmeet Singh} and Krystalee Wiebe and Jennifer Ens and Andrii Fatiukha and Liubov Govta and Tzion Fahima and Pozniak, {Curtis J.}",

note = "Funding Information: The authors are thankful to Dr. Xianming Chen (USDA) and Dr. Randy Kutcher (University of Saskatchewan) for making stripe rust races available, and to USDA-ARS National Small Grains Collection and Dr. Peng Zhang (University of Sydney) for providing seed material. The authors are grateful to Madison Kist, Xue (Snow) Lin, Lexie Gerl, Ayla Lichtenwald and Maureen Troesch for their technical support. This work was conducted through the 4D Wheat Project (C.J.P.) supported by Genome Canada, Genome Prairie, the Saskatchewan Ministry of Agriculture, Saskatchewan Wheat Development Commission, Alberta Wheat Development Commission, Secan, Agriculture and Agri-Food Canada and the Manitoba Crop Alliance; the “Maximizing Durable Disease Resistance in Wheat” Project (C.J.P.) sponsored by the Saskatchewan Ministry of Agriculture grant number ADF20200353; the Israel Science Foundation, grant number 1366/18 (T.F.). Funding Information: The authors are thankful to Dr. Xianming Chen (USDA) and Dr. Randy Kutcher (University of Saskatchewan) for making stripe rust races available, and to USDA-ARS National Small Grains Collection and Dr. Peng Zhang (University of Sydney) for providing seed material. The authors are grateful to Madison Kist, Xue (Snow) Lin, Lexie Gerl, Ayla Lichtenwald and Maureen Troesch for their technical support. This work was conducted through the 4D Wheat Project (C.J.P.) supported by Genome Canada, Genome Prairie, the Saskatchewan Ministry of Agriculture, Saskatchewan Wheat Development Commission, Alberta Wheat Development Commission, Secan, Agriculture and Agri-Food Canada and the Manitoba Crop Alliance; the “Maximizing Durable Disease Resistance in Wheat” Project (C.J.P.) sponsored by the Saskatchewan Ministry of Agriculture grant number ADF20200353; the Israel Science Foundation, grant number 1366/18 (T.F.). Publisher Copyright: {\textcopyright} 2022, The Author(s).",

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