Differential inhibition by Bacillus thuringiensis δ endotoxin of leucine and aspartic acid uptake into BBMV from midgut of Manduca sexta

Moshe Reuveni; Peter E. Dunn

doi:10.1016/0006-291X(91)92049-P

Differential inhibition by Bacillus thuringiensis δ endotoxin of leucine and aspartic acid uptake into BBMV from midgut of Manduca sexta

Research output: Contribution to journal › Article › peer-review

Abstract

Pre-incubation of brush border membrane vesicles (BBMV) isolated from the midgut of Manduca sexta with activated Bacillus thuringiensis δ edotoxin for a short period resulted in differential inhibition of K⁺-dependent transport of leucine relative to the effect on K⁺-dependent transport of aspartic acid. The difference in I_{1 2} (5 fold greater for aspartic acid than for leucine) is interpreted as the result of enhanced binding of the B. thuringiensis δ endotoxin to the leucine transport system.

Original language	English
Pages (from-to)	1089-1093
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	181
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(91)92049-P
State	Published - 31 Dec 1991
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/0006-291X(91)92049-P

Cite this

@article{f3ec2d4bca314c39bda88014b737c8f3,

title = "Differential inhibition by Bacillus thuringiensis δ endotoxin of leucine and aspartic acid uptake into BBMV from midgut of Manduca sexta",

abstract = "Pre-incubation of brush border membrane vesicles (BBMV) isolated from the midgut of Manduca sexta with activated Bacillus thuringiensis δ edotoxin for a short period resulted in differential inhibition of K+-dependent transport of leucine relative to the effect on K+-dependent transport of aspartic acid. The difference in I 1 2 (5 fold greater for aspartic acid than for leucine) is interpreted as the result of enhanced binding of the B. thuringiensis δ endotoxin to the leucine transport system.",

author = "Moshe Reuveni and Dunn, \{Peter E.\}",

year = "1991",

month = dec,

day = "31",

doi = "10.1016/0006-291X(91)92049-P",

language = "English",

volume = "181",

pages = "1089--1093",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Differential inhibition by Bacillus thuringiensis δ endotoxin of leucine and aspartic acid uptake into BBMV from midgut of Manduca sexta

AU - Reuveni, Moshe

AU - Dunn, Peter E.

PY - 1991/12/31

Y1 - 1991/12/31

N2 - Pre-incubation of brush border membrane vesicles (BBMV) isolated from the midgut of Manduca sexta with activated Bacillus thuringiensis δ edotoxin for a short period resulted in differential inhibition of K+-dependent transport of leucine relative to the effect on K+-dependent transport of aspartic acid. The difference in I 1 2 (5 fold greater for aspartic acid than for leucine) is interpreted as the result of enhanced binding of the B. thuringiensis δ endotoxin to the leucine transport system.

AB - Pre-incubation of brush border membrane vesicles (BBMV) isolated from the midgut of Manduca sexta with activated Bacillus thuringiensis δ edotoxin for a short period resulted in differential inhibition of K+-dependent transport of leucine relative to the effect on K+-dependent transport of aspartic acid. The difference in I 1 2 (5 fold greater for aspartic acid than for leucine) is interpreted as the result of enhanced binding of the B. thuringiensis δ endotoxin to the leucine transport system.

UR - https://www.scopus.com/pages/publications/0026350706

U2 - 10.1016/0006-291X(91)92049-P

DO - 10.1016/0006-291X(91)92049-P

M3 - Article

C2 - 1662492

AN - SCOPUS:0026350706

SN - 0006-291X

VL - 181

SP - 1089

EP - 1093

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Differential inhibition by Bacillus thuringiensis δ endotoxin of leucine and aspartic acid uptake into BBMV from midgut of Manduca sexta

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this