Differential inhibition by Bacillus thuringiensis δ endotoxin of leucine and aspartic acid uptake into BBMV from midgut of Manduca sexta

Moshe Reuveni; Peter E. Dunn

doi:10.1016/0006-291X(91)92049-P

Differential inhibition by Bacillus thuringiensis δ endotoxin of leucine and aspartic acid uptake into BBMV from midgut of Manduca sexta

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Abstract

Pre-incubation of brush border membrane vesicles (BBMV) isolated from the midgut of Manduca sexta with activated Bacillus thuringiensis δ edotoxin for a short period resulted in differential inhibition of K⁺-dependent transport of leucine relative to the effect on K⁺-dependent transport of aspartic acid. The difference in I_{1 2} (5 fold greater for aspartic acid than for leucine) is interpreted as the result of enhanced binding of the B. thuringiensis δ endotoxin to the leucine transport system.

Original language	English
Pages (from-to)	1089-1093
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	181
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(91)92049-P
State	Published - 31 Dec 1991
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(91)92049-P

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AB - Pre-incubation of brush border membrane vesicles (BBMV) isolated from the midgut of Manduca sexta with activated Bacillus thuringiensis δ edotoxin for a short period resulted in differential inhibition of K+-dependent transport of leucine relative to the effect on K+-dependent transport of aspartic acid. The difference in I 1 2 (5 fold greater for aspartic acid than for leucine) is interpreted as the result of enhanced binding of the B. thuringiensis δ endotoxin to the leucine transport system.

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Differential inhibition by Bacillus thuringiensis δ endotoxin of leucine and aspartic acid uptake into BBMV from midgut of Manduca sexta

Abstract

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