Quantitative and functional measurements of G proteins were undertaken in mononuclear leukocytes of bipolar disordered patients comparing bipolar depressed with manic patients groups in order to verify whether any alterations observed in G protein functional or immunoreactive measures in bipolar mood disorder are state- or trait-dependent characteristics. Compared with the control group of 30 subjects, isoproterenol- and carbamylcholine-enhanced Gpp(NH)p binding capacities were highly significantly increased in the group of 20 manic patients, while highly significantly reduced in the group of 11 bipolar depressed patients. While manic patients showed highly significant elevations in mononuclear leukocytes levels of G(αs) and G(αi), evaluated through immunoblot analysis using specific polyclonal antibodies against the subunit proteins, mononuclear leukocytes of bipolar depressed patients show significant reductions in G(αs) and G(αi) immunoreactive levels. G(β) subunit levels were found to be similar in all three groups. The changes in G protein measures observed in mononuclear leukocytes of mood disordered patients thus represent state characteristics of the disorder.
Bibliographical noteFunding Information:
This study was supported by research grants from the Stanley Foundation USA and the Chief Scientist Office of the Israel Ministry of Health to G.S. and S.A., and by the Zeldovich Foundation and Yadgaroff Family Foundation to G.S. S. Avissar is Fellow of the National Institute of Psychobiology in Israel in honor of Prof. Joel Elkes and is recipient of NARSAD Young Investigator Award.
- G proteins bipolar mood disorder
- mononuclear leukocytes
ASJC Scopus subject areas
- Clinical Psychology
- Psychiatry and Mental health