Differences in Monoterpene Biosynthesis and Accumulation in Pistacia palaestina Leaves and Aphid-Induced Galls

Karin Rand, Einat Bar, Matan Ben Ari, Rachel Davidovich-Rikanati, Natalia Dudareva, Moshe Inbar, Efraim Lewinsohn

Research output: Contribution to journalArticlepeer-review

Abstract

Certain insect species can induce gall formation on numerous plants species. Although the mechanism of gall development is largely unknown, it is clear that insects manipulate their hosts’ anatomy, physiology, and chemistry for their own benefit. It is well known that insect-induced galls often contain vast amounts of plant defensive compounds as compared to non-colonized tissues, but it is not clear if defensive compounds can be produced in situ in the galled tissues. To answer this question, we analyzed terpene accumulation patterns and possible independent biosynthetic potential of galls induced by the aphid Baizongia pistaciae L. on the terminal buds of Pistacia palaestina Boiss. We compared monoterpene levels and monoterpene synthase enzyme activity in galls and healthy leaves from individual trees growing in a natural setting. At all developmental stages, monoterpene content and monoterpene synthase activity were consistently (up to 10 fold on a fresh weight basis) higher in galls than in intact non-colonized leaves. A remarkable tree to tree variation in the products produced in vitro from the substrate geranyl diphosphate by soluble protein extracts derived from individual trees was observed. Furthermore, galls and leaves from the same trees displayed enhanced and often distinct biosynthetic capabilities. Our results clearly indicate that galls possess independent metabolic capacities to produce and accumulate monoterpenes as compared to leaves. Our study indicates that galling aphids manipulate the enzymatic machinery of their host plant, intensifying their own defenses against natural enemies.

Original languageEnglish
Pages (from-to)143-152
Number of pages10
JournalJournal of Chemical Ecology
Volume43
Issue number2
DOIs
StatePublished - 1 Feb 2017

Bibliographical note

Funding Information:
This work was supported by the US-Israel Binational Science Foundation Grant No. 2012241. We also thank Dr. Itay Gonda and Dr. Raz Krizevski for their useful comments and advice. Publication Number 92016 from the Volcani Center, Rishon LeZion, Israel.

Publisher Copyright:
© 2017, Springer Science+Business Media New York.

Keywords

  • Defensive compounds
  • Host manipulation
  • Insect galls
  • Monoterpene synthase
  • Monoterpenes

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry

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