Developmental regulation of glial cell phagocytic function during Drosophila embryogenesis

Boris Shklyar; Yael Sellman; Jeny Shklover; Ketty Mishnaevski; Flonia Levy-Adam; Estee Kurant

doi:10.1016/j.ydbio.2014.07.005

Developmental regulation of glial cell phagocytic function during Drosophila embryogenesis

Boris Shklyar, Yael Sellman, Jeny Shklover, Ketty Mishnaevski, Flonia Levy-Adam, Estee Kurant

Research output: Contribution to journal › Article › peer-review

Abstract

The proper removal of superfluous neurons through apoptosis and subsequent phagocytosis is essential for normal development of the central nervous system (CNS). During Drosophila embryogenesis, a large number of apoptotic neurons are efficiently engulfed and degraded by phagocytic glia. Here we demonstrate that glial proficiency to phagocytose relies on expression of phagocytic receptors for apoptotic cells, SIMU and DRPR. Moreover, we reveal that the phagocytic ability of embryonic glia is established as part of a developmental program responsible for glial cell fate determination and is not triggered by apoptosis per se. Explicitly, we provide evidence for a critical role of the major regulators of glial identity, gcm and repo, in controlling glial phagocytic function through regulation of SIMU and DRPR specific expression. Taken together, our study uncovers molecular mechanisms essential for establishment of embryonic glia as primary phagocytes during CNS development.

Original language	English
Pages (from-to)	255-269
Number of pages	15
Journal	Developmental Biology
Volume	393
Issue number	2
DOIs	https://doi.org/10.1016/j.ydbio.2014.07.005
State	Published - 2014
Externally published	Yes

Bibliographical note

Funding Information:
We would like to thank B. Jones, H. Steller, E. Arama, M. Freeman, N. Franc, O. Schuldiner, Hybridoma bank and the Bloomington Stock Center for generously providing fly strains and antibodies. We thank A. Salzberg, Z. Paroush and T. Schultheiss for comments on the manuscript and the Kurant laboratory members for constructive criticism and support. We also thank E. Suss-Toby and L. Liba at the Interdepartmental Bioimaging facility for excellent technical support. We gratefully acknowledge financial support from Israel Science Foundation (Grant no 427/11 ).

Publisher Copyright:
© 2014 Elsevier Inc.

Keywords

Apoptosis
Development
Drosophila
Glia
Phagocytosis
SIMU

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology

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10.1016/j.ydbio.2014.07.005

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title = "Developmental regulation of glial cell phagocytic function during Drosophila embryogenesis",

abstract = "The proper removal of superfluous neurons through apoptosis and subsequent phagocytosis is essential for normal development of the central nervous system (CNS). During Drosophila embryogenesis, a large number of apoptotic neurons are efficiently engulfed and degraded by phagocytic glia. Here we demonstrate that glial proficiency to phagocytose relies on expression of phagocytic receptors for apoptotic cells, SIMU and DRPR. Moreover, we reveal that the phagocytic ability of embryonic glia is established as part of a developmental program responsible for glial cell fate determination and is not triggered by apoptosis per se. Explicitly, we provide evidence for a critical role of the major regulators of glial identity, gcm and repo, in controlling glial phagocytic function through regulation of SIMU and DRPR specific expression. Taken together, our study uncovers molecular mechanisms essential for establishment of embryonic glia as primary phagocytes during CNS development.",

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author = "Boris Shklyar and Yael Sellman and Jeny Shklover and Ketty Mishnaevski and Flonia Levy-Adam and Estee Kurant",

note = "Funding Information: We would like to thank B. Jones, H. Steller, E. Arama, M. Freeman, N. Franc, O. Schuldiner, Hybridoma bank and the Bloomington Stock Center for generously providing fly strains and antibodies. We thank A. Salzberg, Z. Paroush and T. Schultheiss for comments on the manuscript and the Kurant laboratory members for constructive criticism and support. We also thank E. Suss-Toby and L. Liba at the Interdepartmental Bioimaging facility for excellent technical support. We gratefully acknowledge financial support from Israel Science Foundation (Grant no 427/11 ). Publisher Copyright: {\textcopyright} 2014 Elsevier Inc.",

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AU - Sellman, Yael

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AU - Mishnaevski, Ketty

AU - Levy-Adam, Flonia

AU - Kurant, Estee

N1 - Funding Information: We would like to thank B. Jones, H. Steller, E. Arama, M. Freeman, N. Franc, O. Schuldiner, Hybridoma bank and the Bloomington Stock Center for generously providing fly strains and antibodies. We thank A. Salzberg, Z. Paroush and T. Schultheiss for comments on the manuscript and the Kurant laboratory members for constructive criticism and support. We also thank E. Suss-Toby and L. Liba at the Interdepartmental Bioimaging facility for excellent technical support. We gratefully acknowledge financial support from Israel Science Foundation (Grant no 427/11 ). Publisher Copyright: © 2014 Elsevier Inc.

PY - 2014

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N2 - The proper removal of superfluous neurons through apoptosis and subsequent phagocytosis is essential for normal development of the central nervous system (CNS). During Drosophila embryogenesis, a large number of apoptotic neurons are efficiently engulfed and degraded by phagocytic glia. Here we demonstrate that glial proficiency to phagocytose relies on expression of phagocytic receptors for apoptotic cells, SIMU and DRPR. Moreover, we reveal that the phagocytic ability of embryonic glia is established as part of a developmental program responsible for glial cell fate determination and is not triggered by apoptosis per se. Explicitly, we provide evidence for a critical role of the major regulators of glial identity, gcm and repo, in controlling glial phagocytic function through regulation of SIMU and DRPR specific expression. Taken together, our study uncovers molecular mechanisms essential for establishment of embryonic glia as primary phagocytes during CNS development.

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Developmental regulation of glial cell phagocytic function during Drosophila embryogenesis

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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