Abstract
Sixteen monoclonal antibodies (MAbs) specific to 2 urochordate genera (Botryllus schlosseri and Botrylloides) intracellular epitopes were generated in mice immunized with a mixture of fresh and paraformaldehyde-fixed cells obtained from animal's blood and cells from dissociated organs. Hybridoma clones were selected by ELISA tests and immunohistochemistry assays on paraffin-embedded animal tissues. Five MAbs were tested for reactions with different zooidal organs and cell compartments; 7 MAbs were tested, separately, on 5 different botryllid colonies (3 Botryllus and 2 Botrylloides). The results revealed high polymorphism. Whereas some of the MAbs recognized, specifically, only part of the botryllid genotypes tested, others recognized only part of the cellular compartments. These MAbs will be used as an important tool in the study of botryllid ascidian immunology and developmental biology, revealing the first wide panel of MAbs specific to urochordate intracellular antigens.
| Original language | English |
|---|---|
| Pages (from-to) | 190-195 |
| Number of pages | 6 |
| Journal | Cell Biology International |
| Volume | 30 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 2006 |
| Externally published | Yes |
Bibliographical note
Funding Information:We are grateful to Elizabetha Moisseva, Guy Paz and Maria Sigal for their indispensable assistance. This study was supported by a grant from the NIH (R01-DK 54762), by a grant from the Israel Science Foundation and by the US – Israel Binational Science Foundation.
Keywords
- Botrylloides
- Botryllus
- Monoclonal antibodies
ASJC Scopus subject areas
- Cell Biology