Developing novel microsatellite markers by NGS technology for Rhopilema nomadica, an invasive jellyfish

Jacob Douek; Zoya Harbuzov; Bella S. Galil; Baruch Rinkevich

doi:10.1007/s11033-020-05533-0

Developing novel microsatellite markers by NGS technology for Rhopilema nomadica, an invasive jellyfish

Jacob Douek, Zoya Harbuzov, Bella S. Galil, Baruch Rinkevich

Research output: Contribution to journal › Article › peer-review

Abstract

Twelve microsatellite loci, obtained by whole genome sequencing approach, were developed and validated for the rhizostomatid jellyfish Rhopilema nomadica, the most pernicious invasive species in the Mediterranean Sea. A sample of 40 specimens collected at six locations along the Mediterranean coast of Israel were genotyped and all loci presented suitable outcomes to population genetic studies, revealing 5–19 alleles/locus with clean and reproducible amplifications. Observed and expected heterozygosity ranged 0.0.353 to 0.971 and 0.335 to 0.870, respectively, and the fixation index (inbreeding coefficient) and the polymorphic information content (PIC) ranged between − 0.190 and 0.240 and 0.32 to 0.858, respectively. The new set of microsatellite loci will be used to study long-term changes in the population genetic parameters of this invasive species.

Original language	English
Pages (from-to)	4821-4825
Number of pages	5
Journal	Molecular Biology Reports
Volume	47
Issue number	6
DOIs	https://doi.org/10.1007/s11033-020-05533-0
State	Published - 1 Jun 2020
Externally published	Yes

Bibliographical note

Funding Information:
Thanks to O. Klein for his photograph of a Rhopilema swarm. We thank Rinkevich’s and Lubinevsky’s labs members for the help in jellyfish collections. This study was supported by the Israeli Ministry of Energy, contract Nos.: 211–17-023 and-218–17-009

Publisher Copyright:
© 2020, Springer Nature B.V.

Keywords

Invasive species
Israel
Microsatellites
Population genetics
Rhopilema nomadica
Whole genome sequencing

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/s11033-020-05533-0

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title = "Developing novel microsatellite markers by NGS technology for Rhopilema nomadica, an invasive jellyfish",

abstract = "Twelve microsatellite loci, obtained by whole genome sequencing approach, were developed and validated for the rhizostomatid jellyfish Rhopilema nomadica, the most pernicious invasive species in the Mediterranean Sea. A sample of 40 specimens collected at six locations along the Mediterranean coast of Israel were genotyped and all loci presented suitable outcomes to population genetic studies, revealing 5–19 alleles/locus with clean and reproducible amplifications. Observed and expected heterozygosity ranged 0.0.353 to 0.971 and 0.335 to 0.870, respectively, and the fixation index (inbreeding coefficient) and the polymorphic information content (PIC) ranged between − 0.190 and 0.240 and 0.32 to 0.858, respectively. The new set of microsatellite loci will be used to study long-term changes in the population genetic parameters of this invasive species.",

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N1 - Funding Information: Thanks to O. Klein for his photograph of a Rhopilema swarm. We thank Rinkevich’s and Lubinevsky’s labs members for the help in jellyfish collections. This study was supported by the Israeli Ministry of Energy, contract Nos.: 211–17-023 and-218–17-009 Publisher Copyright: © 2020, Springer Nature B.V.

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Developing novel microsatellite markers by NGS technology for Rhopilema nomadica, an invasive jellyfish

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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