Determination of the endonuclease activity encoded by retrotransposone Penelope

Mobile element Penelope is mobilized in the course of hybrid dysgenesis in D. virilis. This element is also responsible for the activation of other unrelated families of TE occurring in the progeny of dysgenic crosses. Penelope elements have extremely variable structure and combine some properties of LINEs and LTR-containing elements. Penelope-like elements (PLEs) have been recently described in various organisms including fish species, rotifers and amoebae. Computer analysis enabled to predict the presence of reverse transcriptase domain in Penelope-encoded polyprotein as well as UvrC type endonuclease at the C-end of the element. It is noteworthy that none of the previously described retroelements was shown to contain such a nuclease. Multiple alignments revealed five conservative catalytic motifs and all conservative residues present in GIY-YIG endonuclease family within Penelope-encoded protein. Herein we have demonstrated that Penelope element isolated from D. virilis encodes functionally active endonuclease exhibiting some sequence-specificity to the sequence previously demonstrated to serve as Penelope genomic insertion site.