Degenerative changes in the A375 melanoma line induced by transforming growth factor beta 1

Sarit Larisch-Bloch; Rena Yarom; Dov Sulitzeanu

doi:10.1016/0165-2478(92)90040-U

Degenerative changes in the A375 melanoma line induced by transforming growth factor beta 1

Sarit Larisch-Bloch, Rena Yarom, Dov Sulitzeanu

Research output: Contribution to journal › Article › peer-review

Abstract

A375 human melanoma cell cultures grown in the presence of TGFβ contained greatly reduced cell numbers and exhibited drastic alterations in cell morphology compared to the control cultures. Preincubation of the cells with the cytokine for only 18 h was sufficient to induce these changes irreversibly. Examination of TGFβ-treated cells in the electron microscope revealed large numbers of lipid-filled vacuoles in the cytoplasm, greatly contracted nuclei and some loss of the otherwise abundant microvilli. Thus TGFβ may have a direct toxic effect on the A375 melanoma cells.

Original language	English
Pages (from-to)	151-156
Number of pages	6
Journal	Immunology Letters
Volume	33
Issue number	2
DOIs	https://doi.org/10.1016/0165-2478(92)90040-U
State	Published - Jul 1992
Externally published	Yes

Bibliographical note

Funding Information:
We gratefully acknowledge the support of The Society of Research Associates of the Lautenberg Center and the Concern Foundation of Los Angeles. We also thank Ms. B. Sterman for techni- cal assistance and special thanks to Ms. Marcella Wachtel and Ms. Inbal Heth for assistance in the preparation of this manuscript.

Keywords

Melanoma cell line
TGFβ

ASJC Scopus subject areas

Immunology and Allergy
Immunology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0165-2478(92)90040-U

Cite this

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title = "Degenerative changes in the A375 melanoma line induced by transforming growth factor beta 1",

abstract = "A375 human melanoma cell cultures grown in the presence of TGFβ contained greatly reduced cell numbers and exhibited drastic alterations in cell morphology compared to the control cultures. Preincubation of the cells with the cytokine for only 18 h was sufficient to induce these changes irreversibly. Examination of TGFβ-treated cells in the electron microscope revealed large numbers of lipid-filled vacuoles in the cytoplasm, greatly contracted nuclei and some loss of the otherwise abundant microvilli. Thus TGFβ may have a direct toxic effect on the A375 melanoma cells.",

keywords = "Melanoma cell line, TGFβ",

author = "Sarit Larisch-Bloch and Rena Yarom and Dov Sulitzeanu",

note = "Funding Information: We gratefully acknowledge the support of The Society of Research Associates of the Lautenberg Center and the Concern Foundation of Los Angeles. We also thank Ms. B. Sterman for techni- cal assistance and special thanks to Ms. Marcella Wachtel and Ms. Inbal Heth for assistance in the preparation of this manuscript.",

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doi = "10.1016/0165-2478(92)90040-U",

language = "English",

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pages = "151--156",

journal = "Immunology Letters",

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T1 - Degenerative changes in the A375 melanoma line induced by transforming growth factor beta 1

AU - Larisch-Bloch, Sarit

AU - Yarom, Rena

AU - Sulitzeanu, Dov

N1 - Funding Information: We gratefully acknowledge the support of The Society of Research Associates of the Lautenberg Center and the Concern Foundation of Los Angeles. We also thank Ms. B. Sterman for techni- cal assistance and special thanks to Ms. Marcella Wachtel and Ms. Inbal Heth for assistance in the preparation of this manuscript.

PY - 1992/7

Y1 - 1992/7

N2 - A375 human melanoma cell cultures grown in the presence of TGFβ contained greatly reduced cell numbers and exhibited drastic alterations in cell morphology compared to the control cultures. Preincubation of the cells with the cytokine for only 18 h was sufficient to induce these changes irreversibly. Examination of TGFβ-treated cells in the electron microscope revealed large numbers of lipid-filled vacuoles in the cytoplasm, greatly contracted nuclei and some loss of the otherwise abundant microvilli. Thus TGFβ may have a direct toxic effect on the A375 melanoma cells.

AB - A375 human melanoma cell cultures grown in the presence of TGFβ contained greatly reduced cell numbers and exhibited drastic alterations in cell morphology compared to the control cultures. Preincubation of the cells with the cytokine for only 18 h was sufficient to induce these changes irreversibly. Examination of TGFβ-treated cells in the electron microscope revealed large numbers of lipid-filled vacuoles in the cytoplasm, greatly contracted nuclei and some loss of the otherwise abundant microvilli. Thus TGFβ may have a direct toxic effect on the A375 melanoma cells.

KW - Melanoma cell line

KW - TGFβ

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JF - Immunology Letters

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Degenerative changes in the A375 melanoma line induced by transforming growth factor beta 1

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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