A375 human melanoma cell cultures grown in the presence of TGFβ contained greatly reduced cell numbers and exhibited drastic alterations in cell morphology compared to the control cultures. Preincubation of the cells with the cytokine for only 18 h was sufficient to induce these changes irreversibly. Examination of TGFβ-treated cells in the electron microscope revealed large numbers of lipid-filled vacuoles in the cytoplasm, greatly contracted nuclei and some loss of the otherwise abundant microvilli. Thus TGFβ may have a direct toxic effect on the A375 melanoma cells.
Bibliographical noteFunding Information:
We gratefully acknowledge the support of The Society of Research Associates of the Lautenberg Center and the Concern Foundation of Los Angeles. We also thank Ms. B. Sterman for techni- cal assistance and special thanks to Ms. Marcella Wachtel and Ms. Inbal Heth for assistance in the preparation of this manuscript.
- Melanoma cell line
ASJC Scopus subject areas
- Immunology and Allergy