TY - JOUR
T1 - Daedalea gibbosasubstances inhibit LPS-induced expression of iNOS by suppression of NF-γB and MAPK activities in RAW 264.7 macrophage cells
AU - Ruimi, Nili
AU - Rwashdeh, Haithem
AU - Wasser, Solomon
AU - Konkimalla, Badireenath
AU - Efferth, Thomas
AU - Bprgatti, Monica
AU - Gambari, Roberto
AU - Mahajna, Jamal
PY - 2010
Y1 - 2010
N2 - Nitric oxide (NO) is a radical molecule produced by iNOS and plays a role in various physiological and pathophysiological conditions including inflammatory diseases and cancer. In the present study, organic extract of Daedalea gibbosa was effective in inhibiting NO and PGE2 production in RAW 264.7 cells. The extract of D. gibbosa was chemically fractionated leading to the isolation of three active fractions (F5-F7) that were effective in inhibiting NO and iNOS production. In addition, F6 and F7 significantly inhibited the iNOS transcript, while F5 did not cause a reduction in the iNOS transcript. Furthermore, the active fractions showed a differential effect on levels of phospho-p38, phospho-JNK, and phospho-IKBα. Phopsho-p38 was moderately inhibited by F5 and only F7 was significantly active in inhibiting phospho-IKBα. Interestingly, all active fractions significantly enhanced levels of phospho-JNK. In addition, the three active fractions also showed differential inhibitory effects on NF-κB DNA binding activity.
AB - Nitric oxide (NO) is a radical molecule produced by iNOS and plays a role in various physiological and pathophysiological conditions including inflammatory diseases and cancer. In the present study, organic extract of Daedalea gibbosa was effective in inhibiting NO and PGE2 production in RAW 264.7 cells. The extract of D. gibbosa was chemically fractionated leading to the isolation of three active fractions (F5-F7) that were effective in inhibiting NO and iNOS production. In addition, F6 and F7 significantly inhibited the iNOS transcript, while F5 did not cause a reduction in the iNOS transcript. Furthermore, the active fractions showed a differential effect on levels of phospho-p38, phospho-JNK, and phospho-IKBα. Phopsho-p38 was moderately inhibited by F5 and only F7 was significantly active in inhibiting phospho-IKBα. Interestingly, all active fractions significantly enhanced levels of phospho-JNK. In addition, the three active fractions also showed differential inhibitory effects on NF-κB DNA binding activity.
KW - IiNOS expression
KW - IγBα phosphorylation
KW - MAPK
KW - NF-γB activation pathway
UR - http://www.scopus.com/inward/record.url?scp=76949105676&partnerID=8YFLogxK
U2 - 10.3892/ijmm_00000361
DO - 10.3892/ijmm_00000361
M3 - Article
C2 - 20127048
AN - SCOPUS:76949105676
SN - 1107-3756
VL - 25
SP - 421
EP - 432
JO - International Journal of Molecular Medicine
JF - International Journal of Molecular Medicine
IS - 3
ER -
