Cytogenetic and Genomic Characterization of a Novel Wheat-Tetraploid Thinopyrum elongatum 1BS·1EL Translocation Line with Stripe Rust Resistance

Dandan Wu, Xin Zhao, Yangqiu Xie, Lingyu Li, Yinghui Li, Wei Zhu, Lili Xu, Yi Wang, Jian Zeng, Yiran Cheng, Lina Sha, Xing Fan, Haigin Zhang, Yonghong Zhou, Huoyang Kang

Research output: Contribution to journalArticlepeer-review

Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is a destructive wheat disease pathogen. Thinopyrum elongatum is a valuable germplasm including diploid, tetraploid, and decaploid with plenty of biotic and abiotic resistance. In a previous study, we generated a stripe rust-resistant wheat-tetraploid Th. elongatum 1E/1D substitution line, K17-841-1. To further apply the wild germplasm for wheat breeding, we selected and obtained a new homozygous wheat-tetraploid Th. elongatum translocation line, T1BS·1EL, using genomic in situ hybridization, fluorescence in situ hybridization (FISH), oligo-FISH painting, and the wheat 55K single nucleotide polymorphism genotyping array. The T1BS·1EL is highly resistant to stripe rust at the seedling and adult stages. Pedigree and molecular marker analyses revealed that the resistance gene was located on the chromosome arm 1EL of tetraploid Th. elongatum, tentatively named Yr1EL. In addition, we developed and validated 32 simple sequence repeat markers and two kompetitive allele-specific PCR assays that were specific to the tetraploid Th. elongatum chromosome arm 1EL to facilitate marker-assisted selection for alien 1EL stripe rust resistance breeding. This will help us explore and locate the stripe rust resistance gene mapping on the 1E chromosome and deploy it in the wheat breeding program.

Original languageEnglish
Pages (from-to)2065-2072
Number of pages8
JournalPlant Disease
Volume108
Issue number7
DOIs
StatePublished - Jul 2024
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2024 The American Phytopathological Society.

Keywords

  • chromosome translocation line
  • molecular marker
  • stripe rust
  • tetraploid Thinopyrum elongatum

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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