We analyzed the molecular selection of a typical subterranean rodent Gansu zokor (Eospalax cansus) combined with mitochondrial cytochrome b (cytb) and D-loop sequences. A total of 121 samples (from 12 sites belonging to 3 main geographical units: U1, U2, and U3) were sequenced, from which 40 cytb haplotypes and 53 D-loop haplotypes were identified. The results of our tests from two site-specific models (M2a and M8) and one branch-site model (Clade model C) suggest that the evolution of cytb in Gansu zokor is chiefly governed by purifying selection. Moreover, the distribution of non-synonymous mutations and the tests by Clade model C indicated the cytb in U3, where the zokors were confronted with more severe hypoxia because of higher altitude and a colder, drier climate, were facing a higher purifying selection pressure than those in U1 and U2.
Bibliographical noteFunding Information:
This study was supported by the General Programs of the National Natural Science Foundation of China (Nos. 31101628 and 30970366 ), the Training Qualified People Plan “Hope of the West China” of the Chinese Academy of Sciences (CAS) (No. O954021211 ), and the CAS President Scholarship (to G. Lin). E. Nevo thanks the Ancel Teicher Research Foundation of Genetics and Molecular Evolution for financial support. We also thank Robin Permut for her assistance in editing.
- Cytochrome B
- Purifying selection
ASJC Scopus subject areas
- Animal Science and Zoology