Culture supernatants of lymphokine-activated killer (LAK) cells contain a high-molecular-weight cytotoxic lymphokine

Sarit Larisch-Bloch; Barbara Sterman; David Weiss; Dov Sulitzeanu

doi:10.1016/0165-2478(89)90150-8

Culture supernatants of lymphokine-activated killer (LAK) cells contain a high-molecular-weight cytotoxic lymphokine

Sarit Larisch-Bloch, Barbara Sterman, David Weiss, Dov Sulitzeanu

Research output: Contribution to journal › Article › peer-review

Abstract

Supernatants of human lymphokine-activated killer (LAK) cells grown in vitro were tested for cytotoxic activity against several mouse and human neoplastic cell lines. All LAK preparations tested (14/14) exhibited cytotoxic activity (40-90% killing of the target cells). Sephacryl S-300 Gel filtration experiments indicated that the biological activity of the LAK supernatant is associated with molecular moities ranging from 800 kDa or more, to less than 10 kDa. The finding of strong cytotoxic activity in LAK supernatants against several tumor lines points to the possibility of employing soluble products of these cells, rather than the living cells themselves, for therapeutic purposes.

Original language	English
Pages (from-to)	27-30
Number of pages	4
Journal	Immunology Letters
Volume	23
Issue number	1
DOIs	https://doi.org/10.1016/0165-2478(89)90150-8
State	Published - Nov 1989
Externally published	Yes

Bibliographical note

Funding Information:
We gratefully acknowledge the support of The Society of Research Associates of the Lautenberg Center, The Concern Foundation, Los Angeles, and the Miriam Jaffe Research Grant from the Israel Cancer Association. We also thank Ms. Judith Blakemore, Cetus Corporation, Emeryville, USA, for a generous gift of recombinant IL-2, and Dr. N. Grover for help with the statistical analysis.

Keywords

Cytotoxic lymphokine
LAK cell

ASJC Scopus subject areas

Immunology and Allergy
Immunology

Access to Document

10.1016/0165-2478(89)90150-8

Cite this

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title = "Culture supernatants of lymphokine-activated killer (LAK) cells contain a high-molecular-weight cytotoxic lymphokine",

abstract = "Supernatants of human lymphokine-activated killer (LAK) cells grown in vitro were tested for cytotoxic activity against several mouse and human neoplastic cell lines. All LAK preparations tested (14/14) exhibited cytotoxic activity (40-90% killing of the target cells). Sephacryl S-300 Gel filtration experiments indicated that the biological activity of the LAK supernatant is associated with molecular moities ranging from 800 kDa or more, to less than 10 kDa. The finding of strong cytotoxic activity in LAK supernatants against several tumor lines points to the possibility of employing soluble products of these cells, rather than the living cells themselves, for therapeutic purposes.",

keywords = "Cytotoxic lymphokine, LAK cell",

author = "Sarit Larisch-Bloch and Barbara Sterman and David Weiss and Dov Sulitzeanu",

note = "Funding Information: We gratefully acknowledge the support of The Society of Research Associates of the Lautenberg Center, The Concern Foundation, Los Angeles, and the Miriam Jaffe Research Grant from the Israel Cancer Association. We also thank Ms. Judith Blakemore, Cetus Corporation, Emeryville, USA, for a generous gift of recombinant IL-2, and Dr. N. Grover for help with the statistical analysis.",

year = "1989",

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doi = "10.1016/0165-2478(89)90150-8",

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AU - Larisch-Bloch, Sarit

AU - Sterman, Barbara

AU - Weiss, David

AU - Sulitzeanu, Dov

N1 - Funding Information: We gratefully acknowledge the support of The Society of Research Associates of the Lautenberg Center, The Concern Foundation, Los Angeles, and the Miriam Jaffe Research Grant from the Israel Cancer Association. We also thank Ms. Judith Blakemore, Cetus Corporation, Emeryville, USA, for a generous gift of recombinant IL-2, and Dr. N. Grover for help with the statistical analysis.

PY - 1989/11

Y1 - 1989/11

N2 - Supernatants of human lymphokine-activated killer (LAK) cells grown in vitro were tested for cytotoxic activity against several mouse and human neoplastic cell lines. All LAK preparations tested (14/14) exhibited cytotoxic activity (40-90% killing of the target cells). Sephacryl S-300 Gel filtration experiments indicated that the biological activity of the LAK supernatant is associated with molecular moities ranging from 800 kDa or more, to less than 10 kDa. The finding of strong cytotoxic activity in LAK supernatants against several tumor lines points to the possibility of employing soluble products of these cells, rather than the living cells themselves, for therapeutic purposes.

AB - Supernatants of human lymphokine-activated killer (LAK) cells grown in vitro were tested for cytotoxic activity against several mouse and human neoplastic cell lines. All LAK preparations tested (14/14) exhibited cytotoxic activity (40-90% killing of the target cells). Sephacryl S-300 Gel filtration experiments indicated that the biological activity of the LAK supernatant is associated with molecular moities ranging from 800 kDa or more, to less than 10 kDa. The finding of strong cytotoxic activity in LAK supernatants against several tumor lines points to the possibility of employing soluble products of these cells, rather than the living cells themselves, for therapeutic purposes.

KW - Cytotoxic lymphokine

KW - LAK cell

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Culture supernatants of lymphokine-activated killer (LAK) cells contain a high-molecular-weight cytotoxic lymphokine

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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