Culture supernatants of lymphokine-activated killer (LAK) cells contain a high-molecular-weight cytotoxic lymphokine

Sarit Larisch-Bloch, Barbara Sterman, David Weiss, Dov Sulitzeanu

Research output: Contribution to journalArticlepeer-review


Supernatants of human lymphokine-activated killer (LAK) cells grown in vitro were tested for cytotoxic activity against several mouse and human neoplastic cell lines. All LAK preparations tested (14/14) exhibited cytotoxic activity (40-90% killing of the target cells). Sephacryl S-300 Gel filtration experiments indicated that the biological activity of the LAK supernatant is associated with molecular moities ranging from 800 kDa or more, to less than 10 kDa. The finding of strong cytotoxic activity in LAK supernatants against several tumor lines points to the possibility of employing soluble products of these cells, rather than the living cells themselves, for therapeutic purposes.

Original languageEnglish
Pages (from-to)27-30
Number of pages4
JournalImmunology Letters
Issue number1
StatePublished - Nov 1989
Externally publishedYes

Bibliographical note

Funding Information:
We gratefully acknowledge the support of The Society of Research Associates of the Lautenberg Center, The Concern Foundation, Los Angeles, and the Miriam Jaffe Research Grant from the Israel Cancer Association. We also thank Ms. Judith Blakemore, Cetus Corporation, Emeryville, USA, for a generous gift of recombinant IL-2, and Dr. N. Grover for help with the statistical analysis.


  • Cytotoxic lymphokine
  • LAK cell

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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