Coral Tissue Regeneration and Growth Is Associated with the Presence of Stem-like Cells

Jonathan Levanoni, Amalia Rosner, Ziva Lapidot, Guy Paz, Baruch Rinkevich

Research output: Contribution to journalArticlepeer-review

Abstract

Members of the Cnidaria phylum were studied for centuries to depict the source of their unprecedented regeneration capacity. Although adult stem cells (ASCs) have been recognized in tissue growth/regeneration in many hydrozoans, there has not been any evidence of them in the ancestral Anthozoa class. This study sheds light on the development of epidermal epithelium expansion, akin to blastema, during tissue regeneration after small circular incisions (each 2.77 mm2) and during the natural expansion of tissue across a flat surface in the scleractinian coral Stylophora pistillata. Regeneration was completed within 9 days in 84.5% (n = 64) of the assays. About 35% of the samples regrew a single polyp, 60% showed no polyp regrowth, and approximately 6% exhibited multiple new polyps. We further used histological staining, pH3, Piwi immuno-histochemistry, and qPCR for eight stemness markers: Piwi-1, Nanos-1, Nanos-1-like, Tudor-5, Tudor-7, Boule, Sox-2, and Myc-1. The results revealed the formation of an “addendum”, an epidermal epithelium in the growing edges (in regenerating and normal-growing fronts) inhabited by a cluster of small cells featuring dense nuclei, resembling ASCs, many expressing pH3 as well as Piwi proteins. Most of the stemness genes tested were upregulated. These results indicate the participation of ASCs-like cells in tissue regeneration and growth in scleractinian corals.

Original languageEnglish
Article number343
JournalJournal of Marine Science and Engineering
Volume12
Issue number2
DOIs
StatePublished - Feb 2024
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2024 by the authors.

Keywords

  • Anthozoa
  • ASCs
  • Cnidaria
  • coral
  • coral nubbins
  • stem cell
  • Stylophora pistillata
  • tissue growth
  • tissue regeneration
  • wound healing

ASJC Scopus subject areas

  • Civil and Structural Engineering
  • Water Science and Technology
  • Ocean Engineering

Fingerprint

Dive into the research topics of 'Coral Tissue Regeneration and Growth Is Associated with the Presence of Stem-like Cells'. Together they form a unique fingerprint.

Cite this