Cooperativity, specificity, and evolutionary stability of polycomb targeting in Drosophila

Bernd Schuettengruber, Noa Oded Elkayam, Tom Sexton, Marianne Entrevan, Shani Stern, Aubin Thomas, Eitan Yaffe, Hugues Parrinello, Amos Tanay, Giacomo Cavalli

Research output: Contribution to journalArticlepeer-review

Abstract

Metazoan genomes are partitioned into modular chromosomal domains containing active or repressive chromatin. In flies, Polycomb group (PcG) response elements (PREs) recruit PHO and other DNA-binding factors and act as nucleation sites for the formation of Polycomb repressive domains. The sequence specificity of PREs is not well understood. Here, we use comparative epigenomics and transgenic assays to show that Drosophila domain organization and PRE specification are evolutionarily conserved despite significant cis-element divergence within Polycomb domains, whereas cis-element evolution is strongly correlated with transcription factor binding divergence outside of Polycomb domains. Cooperative interactions of PcG complexes and their recruiting factor PHO stabilize PHO recruitment to low-specificity sequences. Consistently, PHO recruitment to sites within Polycomb domains is stabilized by PRC1. These data suggest that cooperative rather than hierarchical interactions among low-affinity sequences, DNA-binding factors, and the Polycomb machinery are giving rise to specific and strongly conserved 3D structures in Drosophila.

Original languageEnglish
Pages (from-to)219-233
Number of pages15
JournalCell Reports
Volume9
Issue number1
DOIs
StatePublished - 9 Oct 2014
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2014 The Authors.

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

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