TY - JOUR
T1 - Converting of nuisance cyanobacterial biomass to feedstock for bioethanol production by regulation of intracellular carbon flow
T2 - Killing two birds with one stone
AU - Huang, Yingying
AU - Chen, Xuechu
AU - Liu, Silu
AU - Lu, Jinzhong
AU - Shen, Yingshi
AU - Li, Lei
AU - Peng, Lin
AU - Hong, Jingjie
AU - Zhang, Qiuzhuo
AU - Ostrovsky, Ilia
N1 - Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/10
Y1 - 2021/10
N2 - Outbreaks of cyanobacterial harmful algal blooms present a serious threat to ecosystem safety and human well-being. Removal of toxic cyanobacterial biomass from freshwaters and its safe utilization is a worldwide problem. We showed that regulation of intracellular carbon flow by applying nitrogen deprivation strategy with light and inorganic carbon manipulation during the cultivation of cyanobacterium Microcystis allows efficiently convert its biomass into the biofuel feedstock. The proteomic analysis demonstrated that highly efficient feedstock production can be achieved during Microcystis culturing by nitrogen deprivation, which slows down the catabolic consumption of carbohydrates in cells. Further, we developed a novel approach that allows converting the natural Microcystis biomass into carbohydrate-rich feedstock by maintaining a high rate of carbohydrate production at minimal biomass losses. The yield attains 0.22 g ethanol per gram dry biomass, which is in the range of the ethanol yield of sugarcane, but higher than the values of sugarcane waste. The suggested approach provides cost-effective technology for sustainable green energy production from nuisance cyanobacterial biomass. The massive removal of toxic cyanobacterial biomass for bioethanol productions can mitigate the harmful cyanobacterial blooms in eutrophic lakes, and positively affect their restoration.
AB - Outbreaks of cyanobacterial harmful algal blooms present a serious threat to ecosystem safety and human well-being. Removal of toxic cyanobacterial biomass from freshwaters and its safe utilization is a worldwide problem. We showed that regulation of intracellular carbon flow by applying nitrogen deprivation strategy with light and inorganic carbon manipulation during the cultivation of cyanobacterium Microcystis allows efficiently convert its biomass into the biofuel feedstock. The proteomic analysis demonstrated that highly efficient feedstock production can be achieved during Microcystis culturing by nitrogen deprivation, which slows down the catabolic consumption of carbohydrates in cells. Further, we developed a novel approach that allows converting the natural Microcystis biomass into carbohydrate-rich feedstock by maintaining a high rate of carbohydrate production at minimal biomass losses. The yield attains 0.22 g ethanol per gram dry biomass, which is in the range of the ethanol yield of sugarcane, but higher than the values of sugarcane waste. The suggested approach provides cost-effective technology for sustainable green energy production from nuisance cyanobacterial biomass. The massive removal of toxic cyanobacterial biomass for bioethanol productions can mitigate the harmful cyanobacterial blooms in eutrophic lakes, and positively affect their restoration.
KW - Bioenergy production
KW - Carbohydrate
KW - Carbon flows
KW - Cyanobacterial blooms
KW - Renewable resources
UR - http://www.scopus.com/inward/record.url?scp=85109028491&partnerID=8YFLogxK
U2 - 10.1016/j.rser.2021.111364
DO - 10.1016/j.rser.2021.111364
M3 - Article
AN - SCOPUS:85109028491
SN - 1364-0321
VL - 149
JO - Renewable and Sustainable Energy Reviews
JF - Renewable and Sustainable Energy Reviews
M1 - 111364
ER -