Comparison of fluorescence microscopy and different growth media culture methods for acanthamoeba keratitis diagnosis

Avi Peretz, Yuval Geffen, Soergiu D. Socea, Nina Pastukh, Shmuel Graffi

Research output: Contribution to journalArticlepeer-review

Abstract

Acanthamoeba keratitis (AK), a potentially blinding infection of the cornea, is caused by a free-living protozoan. Culture and microscopic examination of corneal scraping tissue material is the conventional method for identifying Acanthamoeba. In this article, we compared several methods for AK diagnosis of 32 patients: microscopic examination using fluorescent dye, specific culture on growth media-non-nutrient agar (NNA), culture on liquid growth media-peptone yeast glucose (PYG), and TYI-S-33. AK was found in 14 patients. Thirteen of the specimens were found AK positive by fluorescence microscopic examination, 11 specimens were found AK positive on PYG growth media, and 9 specimens were found AK positive on TYI-S-33 growth media. Only five specimens were found AK positive on NNA growth media. Therefore, we recommend using fluorescence microscopy technique and culture method, especially PYG liquid media.

Original languageEnglish
Pages (from-to)316-318
Number of pages3
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume93
Issue number2
DOIs
StatePublished - 1 Aug 2015
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2015 by The American Society of Tropical Medicine and Hygiene.

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

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