Comparative transcriptome profile of the leaf elongation zone of wild barley (Hordeum spontaneum) eibi1 mutant and its isogenic wild type

Qin Zhou, Aidong Wang, Ruijun Duan, Jun Yan, Gang Zhao, Eviatar Nevo, Guoxiong Chen

Research output: Contribution to journalArticlepeer-review

Abstract

The naturally occurring wild barley mutant eibi1/hvabcg31 suffers from severe water loss due to the permeable leaf cuticle. Eibi1/HvABCG31 encodes a full ATP-binding cassette (ABC) transporter, HvABCG31, playing a role in cutin deposition in the elongation zone of growing barley leaves. The eibi1 allele has pleiotropic effects on the appearance of leaves, plant stature, fertility, spike and grain size, and rate of germination. Comparative transcriptome profile of the leaf elongation zone of the eibi1 mutant as well as its isogenic wild type showed that various pathogenesis-related genes were up-regulated in the eibi1 mutant. The known cuticle-related genes that we analyzed did not show significant expression difference between the mutant and wild type. These results suggest that the pleiotropic effects may be a compensatory consequence of the activation of defense genes in the eibi1 mutation. Furthermore, we were able to find the mutation of the eibi1/hvabcg31 allele by comparing transcript sequences, which indicated that the RNA-Seq is useful not only for researches on general molecular mechanism but also for the identification of possible mutant genes.

Original languageEnglish
Pages (from-to)834-843
Number of pages10
JournalGenetics and Molecular Biology
Volume40
Issue number4
DOIs
StatePublished - 1 Oct 2017

Bibliographical note

Funding Information:
This work was supported by grants from the National Natural Science Foundation of China (41621001 and 31170369), the Key Project of the Chinese National Programs for Fundamental Research and Development (grant number 2013CB429904), the China-Israel cooperation program grants from the Ministry of Science and Technology in China (grant number 2013DFA32200). We thank BGI Inc. for contributing to the RNA-Seq analysis, and Mrs. Yan Ma for maintaining plant samples, as well as other helpful contributions.

Publisher Copyright:
© 2017, Sociedade Brasileira de Genética. Printed in Brazil.

Keywords

  • ABC transporter
  • Defense genes
  • Desiccation tolerance
  • Plant cuticle
  • RNA-Seq

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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