Cold seep biogenic carbonate crust in the Levantine basin is inhabited by burrowing Phascolosoma aff. turnerae, a sipunculan worm hosting a distinctive microbiota

Maxim Rubin-Blum; Eli Shemesh; Beverly Goodman-Tchernov; Dwight F. Coleman; Zvi Ben-Avraham; Dan Tchernov

doi:10.1016/j.dsr.2014.04.014

Cold seep biogenic carbonate crust in the Levantine basin is inhabited by burrowing Phascolosoma aff. turnerae, a sipunculan worm hosting a distinctive microbiota

Maxim Rubin-Blum, Eli Shemesh, Beverly Goodman-Tchernov, Dwight F. Coleman, Zvi Ben-Avraham, Dan Tchernov

Research output: Contribution to journal › Article › peer-review

Abstract

Biogenic calcium carbonate crusts represent a cryptic habitat that is often associated with hydrocarbon seeps. Most biological observations of these crusts concern the external surfaces and the fauna inhabiting their inner cavities are generally neglected. Exposed carbonates in areas of active seepage at the 1100-m-deep base of the Palmachim slumping feature in the Levantine basin are intensively burrowed by metazoans, especially by sipunculans (peanut worms), identified by genetic and morphological markers as a potentially novel Phascolosoma sp., closely related to Phascolosoma turnerae ( Rice, 1985) and named here P. aff. turnerae. Bacterial 16S-based tag encoded FLX amplicon pyrosequencing (bTEFAP) was utilized to analyze the bacterial community associated with P. aff. turnerae. We compared the bacterial community structure in P. aff. turnerae to the bacterial community structure associated with the sediment-water interface in adjacent gas seeps and in biofilm covering the carbonate crust hosting the sipunculan. A distinctive microbiota, capable of chemosynthesis and sulfide detoxification, was found in association with P. aff. turnerae.

Original language	English
Pages (from-to)	17-26
Number of pages	10
Journal	Deep-Sea Research Part I: Oceanographic Research Papers
Volume	90
Issue number	1
DOIs	https://doi.org/10.1016/j.dsr.2014.04.014
State	Published - Aug 2014

Bibliographical note

Funding Information:
We thank the European Commission FP7 Research Infrastructure Initiative Program “Assemble 227799” for partial support of this project. This research used samples and data provided by the E/V Nautilus Exploration Program, expedition NA019. The authors would like to thank all of the individuals who helped during the expedition, including onboard technical and scientific personnel, the captain and crew of E/V Nautilus . Special thanks to Prof. Anja Schulze from the Texas A&M University at Galveston for fruitful sipunculan phylogeny discussion. We also thank the reviewers for constructive comments on an earlier version of this manuscript.

Keywords

BTEFAP
Cold seep
Cryptic
E/V Nautilus
Mediterranean
Sipuncula

ASJC Scopus subject areas

Oceanography
Aquatic Science

Access to Document

10.1016/j.dsr.2014.04.014

Cite this

Rubin-Blum, M., Shemesh, E., Goodman-Tchernov, B., Coleman, D. F., Ben-Avraham, Z., & Tchernov, D. (2014). Cold seep biogenic carbonate crust in the Levantine basin is inhabited by burrowing Phascolosoma aff. turnerae, a sipunculan worm hosting a distinctive microbiota. Deep-Sea Research Part I: Oceanographic Research Papers, 90(1), 17-26. https://doi.org/10.1016/j.dsr.2014.04.014

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title = "Cold seep biogenic carbonate crust in the Levantine basin is inhabited by burrowing Phascolosoma aff. turnerae, a sipunculan worm hosting a distinctive microbiota",

abstract = "Biogenic calcium carbonate crusts represent a cryptic habitat that is often associated with hydrocarbon seeps. Most biological observations of these crusts concern the external surfaces and the fauna inhabiting their inner cavities are generally neglected. Exposed carbonates in areas of active seepage at the 1100-m-deep base of the Palmachim slumping feature in the Levantine basin are intensively burrowed by metazoans, especially by sipunculans (peanut worms), identified by genetic and morphological markers as a potentially novel Phascolosoma sp., closely related to Phascolosoma turnerae ( Rice, 1985) and named here P. aff. turnerae. Bacterial 16S-based tag encoded FLX amplicon pyrosequencing (bTEFAP) was utilized to analyze the bacterial community associated with P. aff. turnerae. We compared the bacterial community structure in P. aff. turnerae to the bacterial community structure associated with the sediment-water interface in adjacent gas seeps and in biofilm covering the carbonate crust hosting the sipunculan. A distinctive microbiota, capable of chemosynthesis and sulfide detoxification, was found in association with P. aff. turnerae.",

keywords = "BTEFAP, Cold seep, Cryptic, E/V Nautilus, Mediterranean, Sipuncula",

author = "Maxim Rubin-Blum and Eli Shemesh and Beverly Goodman-Tchernov and Coleman, {Dwight F.} and Zvi Ben-Avraham and Dan Tchernov",

note = "Funding Information: We thank the European Commission FP7 Research Infrastructure Initiative Program “Assemble 227799” for partial support of this project. This research used samples and data provided by the E/V Nautilus Exploration Program, expedition NA019. The authors would like to thank all of the individuals who helped during the expedition, including onboard technical and scientific personnel, the captain and crew of E/V Nautilus . Special thanks to Prof. Anja Schulze from the Texas A&M University at Galveston for fruitful sipunculan phylogeny discussion. We also thank the reviewers for constructive comments on an earlier version of this manuscript. ",

year = "2014",

month = aug,

doi = "10.1016/j.dsr.2014.04.014",

language = "English",

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journal = "Deep-Sea Research Part I: Oceanographic Research Papers",

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Rubin-Blum, M, Shemesh, E, Goodman-Tchernov, B, Coleman, DF, Ben-Avraham, Z & Tchernov, D 2014, 'Cold seep biogenic carbonate crust in the Levantine basin is inhabited by burrowing Phascolosoma aff. turnerae, a sipunculan worm hosting a distinctive microbiota', Deep-Sea Research Part I: Oceanographic Research Papers, vol. 90, no. 1, pp. 17-26. https://doi.org/10.1016/j.dsr.2014.04.014

Cold seep biogenic carbonate crust in the Levantine basin is inhabited by burrowing Phascolosoma aff. turnerae, a sipunculan worm hosting a distinctive microbiota. / Rubin-Blum, Maxim; Shemesh, Eli; Goodman-Tchernov, Beverly et al.
In: Deep-Sea Research Part I: Oceanographic Research Papers, Vol. 90, No. 1, 08.2014, p. 17-26.

Research output: Contribution to journal › Article › peer-review

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AU - Rubin-Blum, Maxim

AU - Shemesh, Eli

AU - Goodman-Tchernov, Beverly

AU - Coleman, Dwight F.

AU - Ben-Avraham, Zvi

AU - Tchernov, Dan

N1 - Funding Information: We thank the European Commission FP7 Research Infrastructure Initiative Program “Assemble 227799” for partial support of this project. This research used samples and data provided by the E/V Nautilus Exploration Program, expedition NA019. The authors would like to thank all of the individuals who helped during the expedition, including onboard technical and scientific personnel, the captain and crew of E/V Nautilus . Special thanks to Prof. Anja Schulze from the Texas A&M University at Galveston for fruitful sipunculan phylogeny discussion. We also thank the reviewers for constructive comments on an earlier version of this manuscript.

PY - 2014/8

Y1 - 2014/8

N2 - Biogenic calcium carbonate crusts represent a cryptic habitat that is often associated with hydrocarbon seeps. Most biological observations of these crusts concern the external surfaces and the fauna inhabiting their inner cavities are generally neglected. Exposed carbonates in areas of active seepage at the 1100-m-deep base of the Palmachim slumping feature in the Levantine basin are intensively burrowed by metazoans, especially by sipunculans (peanut worms), identified by genetic and morphological markers as a potentially novel Phascolosoma sp., closely related to Phascolosoma turnerae ( Rice, 1985) and named here P. aff. turnerae. Bacterial 16S-based tag encoded FLX amplicon pyrosequencing (bTEFAP) was utilized to analyze the bacterial community associated with P. aff. turnerae. We compared the bacterial community structure in P. aff. turnerae to the bacterial community structure associated with the sediment-water interface in adjacent gas seeps and in biofilm covering the carbonate crust hosting the sipunculan. A distinctive microbiota, capable of chemosynthesis and sulfide detoxification, was found in association with P. aff. turnerae.

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KW - Cold seep

KW - Cryptic

KW - E/V Nautilus

KW - Mediterranean

KW - Sipuncula

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M3 - Article

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SN - 0967-0637

VL - 90

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JO - Deep-Sea Research Part I: Oceanographic Research Papers

JF - Deep-Sea Research Part I: Oceanographic Research Papers

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Cold seep biogenic carbonate crust in the Levantine basin is inhabited by burrowing Phascolosoma aff. turnerae, a sipunculan worm hosting a distinctive microbiota

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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