Biogenic calcium carbonate crusts represent a cryptic habitat that is often associated with hydrocarbon seeps. Most biological observations of these crusts concern the external surfaces and the fauna inhabiting their inner cavities are generally neglected. Exposed carbonates in areas of active seepage at the 1100-m-deep base of the Palmachim slumping feature in the Levantine basin are intensively burrowed by metazoans, especially by sipunculans (peanut worms), identified by genetic and morphological markers as a potentially novel Phascolosoma sp., closely related to Phascolosoma turnerae ( Rice, 1985) and named here P. aff. turnerae. Bacterial 16S-based tag encoded FLX amplicon pyrosequencing (bTEFAP) was utilized to analyze the bacterial community associated with P. aff. turnerae. We compared the bacterial community structure in P. aff. turnerae to the bacterial community structure associated with the sediment-water interface in adjacent gas seeps and in biofilm covering the carbonate crust hosting the sipunculan. A distinctive microbiota, capable of chemosynthesis and sulfide detoxification, was found in association with P. aff. turnerae.
|Number of pages||10|
|Journal||Deep-Sea Research Part I: Oceanographic Research Papers|
|State||Published - Aug 2014|
Bibliographical noteFunding Information:
We thank the European Commission FP7 Research Infrastructure Initiative Program “Assemble 227799” for partial support of this project. This research used samples and data provided by the E/V Nautilus Exploration Program, expedition NA019. The authors would like to thank all of the individuals who helped during the expedition, including onboard technical and scientific personnel, the captain and crew of E/V Nautilus . Special thanks to Prof. Anja Schulze from the Texas A&M University at Galveston for fruitful sipunculan phylogeny discussion. We also thank the reviewers for constructive comments on an earlier version of this manuscript.
- Cold seep
- E/V Nautilus
ASJC Scopus subject areas
- Aquatic Science