TY - JOUR
T1 - Co-Occurrence of Francisella, Spotted Fever Group Rickettsia, and Midichloria in Avian-Associated Hyalomma rufipes
AU - Hoffman, Tove
AU - Sjödin, Andreas
AU - Öhrman, Caroline
AU - Karlsson, Linda
AU - McDonough, Ryelan Francis
AU - Sahl, Jason W.
AU - Birdsell, Dawn
AU - Wagner, David M.
AU - Carra, Laura G.
AU - Wilhelmsson, Peter
AU - Pettersson, John H.O.
AU - Barboutis, Christos
AU - Figuerola, Jordi
AU - Onrubia, Alejandro
AU - Kiat, Yosef
AU - Piacentini, Dario
AU - Jaenson, Thomas G.T.
AU - Lindgren, Per Eric
AU - Moutailler, Sara
AU - Fransson, Thord
AU - Forsman, Mats
AU - Nilsson, Kenneth
AU - Lundkvist, Åke
AU - Olsen, Björn
N1 - Publisher Copyright:
© 2022 by the authors.
PY - 2022/7/11
Y1 - 2022/7/11
N2 - The migratory behavior of wild birds contributes to the geographical spread of ticks and their microorganisms. In this study, we aimed to investigate the dispersal and co-occurrence of Francisella and spotted fever group Rickettsia (SFGR) in ticks infesting birds migrating northward in the African-Western Palaearctic region (AWPR). Birds were trapped with mist nests across the Mediterranean basin during the 2014 and 2015 spring migration. In total, 575 ticks were collected from 244 birds. We screened the ticks for the species Francisella tularensis, the genus Francisella, and SFGR by microfluidic real-time PCR. Confirmatory analyses and metagenomic sequencing were performed on tick samples that putatively tested positive for F. tularensis during initial screenings. Hyalomma rufipes was the most common tick species and had a high prevalence of Francisella, including co-occurrence of Francisella and SFGR. Metagenomic analysis of total DNA extracted from two H. rufipes confirmed the presence of Francisella, Rickettsia, and Midichloria. Average nucleotide identity and phylogenetic inference indicated the highest identity of the metagenome-assembled genomes to a Francisella-like endosymbiont (FLE), Rickettsia aeschlimannii, and Midichloria mitochondrii. The results of this study suggest that (i) FLE- and SFGR-containing ticks are dispersed by northbound migratory birds in the AWPR, (ii) H. rufipes likely is not involved in transmission of F. tularensis in the AWPR, and (iii) a dual endosymbiosis of FLEs and Midichloria may support some of the nutritional requirements of H. rufipes.
AB - The migratory behavior of wild birds contributes to the geographical spread of ticks and their microorganisms. In this study, we aimed to investigate the dispersal and co-occurrence of Francisella and spotted fever group Rickettsia (SFGR) in ticks infesting birds migrating northward in the African-Western Palaearctic region (AWPR). Birds were trapped with mist nests across the Mediterranean basin during the 2014 and 2015 spring migration. In total, 575 ticks were collected from 244 birds. We screened the ticks for the species Francisella tularensis, the genus Francisella, and SFGR by microfluidic real-time PCR. Confirmatory analyses and metagenomic sequencing were performed on tick samples that putatively tested positive for F. tularensis during initial screenings. Hyalomma rufipes was the most common tick species and had a high prevalence of Francisella, including co-occurrence of Francisella and SFGR. Metagenomic analysis of total DNA extracted from two H. rufipes confirmed the presence of Francisella, Rickettsia, and Midichloria. Average nucleotide identity and phylogenetic inference indicated the highest identity of the metagenome-assembled genomes to a Francisella-like endosymbiont (FLE), Rickettsia aeschlimannii, and Midichloria mitochondrii. The results of this study suggest that (i) FLE- and SFGR-containing ticks are dispersed by northbound migratory birds in the AWPR, (ii) H. rufipes likely is not involved in transmission of F. tularensis in the AWPR, and (iii) a dual endosymbiosis of FLEs and Midichloria may support some of the nutritional requirements of H. rufipes.
KW - African-Western Palaearctic region
KW - Francisella
KW - Francisella-like endosymbionts
KW - Hyalomma rufipes
KW - Midichloria
KW - PCR
KW - Rickettsia aeschlimannii
KW - metagenomics
KW - migratory birds
KW - spotted fever group Rickettsia
KW - ticks
UR - http://www.scopus.com/inward/record.url?scp=85137209919&partnerID=8YFLogxK
U2 - 10.3390/microorganisms10071393
DO - 10.3390/microorganisms10071393
M3 - Article
AN - SCOPUS:85137209919
SN - 2076-2607
VL - 10
JO - Microorganisms
JF - Microorganisms
IS - 7
M1 - 1393
ER -