Yellow rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a devastating fungal disease threatening much of global wheat production. Race-specific resistance (R)-genes are used to control rust diseases, but the rapid emergence of virulent Pst races has prompted the search for a more durable resistance. Here, we report the cloning of Yr15, a broad-spectrum R-gene derived from wild emmer wheat, which encodes a putative kinase-pseudokinase protein, designated as wheat tandem kinase 1, comprising a unique R-gene structure in wheat. The existence of a similar gene architecture in 92 putative proteins across the plant kingdom, including the barley RPG1 and a candidate for Ug8, suggests that they are members of a distinct family of plant proteins, termed here tandem kinase-pseudokinases (TKPs). The presence of kinase-pseudokinase structure in both plant TKPs and the animal Janus kinases sheds light on the molecular evolution of immune responses across these two kingdoms.
Bibliographical noteFunding Information:
This research was supported by the European Community Framework projects BioEx-ploit (CT-2005-513949) and TriticeaeGenome (FP7-212019), the COST Actions TritiGen (FA0604), SUSTAIN (FA1208), and Impact of Nuclear Domains on Gene Expression and Plant Traits (INDEPTH, CA16212), the CSC-IT Center for Science (Finland), the Israel Science Foundation (1719/08), and the US-Israel Binational Agricultural Research and Development Fund (IS-4628-13, US-4916-16). We thank the International Wheat Genome Sequencing Consortium (IWGSC) for providing access to unpublished wheat genome sequences and the Global Yellow Rust Gene Bank for providing Pst races. We also thank P. Auvinen, P.C. Bailey, S. Barinova, H. Belcram, A. Dahan, Z. Gerechter-Amitai, A. Grama, M. Iso-Kokkila, T. Kis-Papo, D. Lewinsohn, U.S. Lönnqvist, A. Malik, Y. Manisterski, I. Manov, H. Miah, A.-M. Narvanto, M.L. Neufeld, K. Neufeld, Y. Ronin, O. Rybak, J. Shanir, N. Sher, O.-P. Smolander, and J. Tanskanen.
© 2018, The Author(s).
ASJC Scopus subject areas
- Chemistry (all)
- Biochemistry, Genetics and Molecular Biology (all)
- Physics and Astronomy (all)