Classification of wheat low-molecular-weight glutenin subunit genes and its chromosome assignment by developing LMW-GS group-specific primers

Hai Long, Yu Ming Wei, Ze Hong Yan, Bernard Baum, Eviatar Nevo, You Liang Zheng

Research output: Contribution to journalArticlepeer-review

Abstract

On the basis of sequence analysis, 69 known low-molecular-weight glutenin subunit (LMW-GS) genes were experimentally classified into nine groups by the deduced amino acid sequence of the highly conserved N-terminal domain. To clarify the chromosomal locations of these groups, 11 specific primer sets were designed to carry out polymerase chain reactions (PCR) with the genomic DNA of group 1 ditelosomic lines of Chinese Spring, among which nine primer sets proved to be LMW-GS group-specific. Each group of LMW-GS genes was specifically assigned on a single chromosome arm and hence to a specific locus. Therefore, these results provided the possibility to predict the chromosome location of a new LMW-GS gene based on its deduced N-terminal sequence. The validity of the classification was confirmed by the amplifications in 27 diploid wheat and Aegilops accessions. The length polymorphisms of LMW-GS genes of groups 1 and 2, and groups 3 and 4.1 were detected in diploid A-genome and S-genome accessions, respectively. The diploid wheat and Aegilops species could be used as valuable resources of novel allele variations of LMW-GS gene in the improvement of wheat quality. The nine LMW-GS group-specific primer sets could be utilized to select specific allele variations of LMW-GS genes in the marker-assisted breeding.

Original languageEnglish
Pages (from-to)1251-1259
Number of pages9
JournalTheoretical And Applied Genetics
Volume111
Issue number7
DOIs
StatePublished - Nov 2005

Keywords

  • Aegilops
  • Alignment analysis
  • Length polymorphism
  • N-terminal domain
  • Triticum aestivum

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Biotechnology

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