Chryseobacterium bovis sp. nov., isolated from raw cow's milk

Elionora Hantsis-Zacharov, Yigal Senderovich, Malka Halpern

Research output: Contribution to journalArticlepeer-review

Abstract

Three Gram-negative, rod-shaped, oxidase-positive, aerobic, non-motile bacterial strains, designated H9T, H10 and H15, were isolated during a study on the diversity of culturable psychrotolerant bacteria in raw cow's milk. Comparisons of 16S rRNA gene sequences showed that the three strains were very closely related to each other (sequence similarities of 99.6-99.8%). A polyphasic taxonomic study of the isolates resulted in their identification as members of the genus Chryseobacterium (family Flavobacteriaceae, phylum Bacteroidetes). The three strains showed ≤96.9% sequence similarity with respect to the type strains of described Chryseobacterium species, indicating that H9T, H10 and H15 represent a novel species of the genus Chryseobacterium. The three strains grew at 7-37 °C (strain H10 grew at up to 41 °C), with 0-2.5% NaCl and at pH 5.0-9.8. The dominant cellular fatty acids of strain H9T were 15:0 iso (38.9%), 15:0 anteiso (15.6%) and 17:0 iso 3-OH (12.7%). Strain H10 also possessed 17:1 iso ω9c (14.8%) as a major fatty acid. On the basis of phenotypic properties and phylogenetic distinctiveness, the three milk isolates represent a novel species in the genus Chryseobacterium, for which the name Chryseobacterium bovis sp. nov. is proposed. The type strain is H9T (=LMG 24227T =DSM 19482T).

Original languageEnglish
Pages (from-to)1024-1028
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume58
Issue number4
DOIs
StatePublished - Apr 2008

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Microbiology

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