Chromosome-based survey sequencing reveals the genome organization of wild wheat progenitor Triticum dicoccoides

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Abstract

Wild emmer wheat (Triticum turgidum ssp. dicoccoides) is the progenitor of wheat. We performed chromosome-based survey sequencing of the 14 chromosomes, examining repetitive sequences, protein-coding genes, miRNA/target pairs and tRNA genes, as well as syntenic relationships with related grasses. We found considerable differences in the content and distribution of repetitive sequences between the A and B subgenomes. The gene contents of individual chromosomes varied widely, not necessarily correlating with chromosome size. We catalogued candidate agronomically important loci, along with new alleles and flanking sequences that can be used to design exome sequencing. Syntenic relationships and virtual gene orders revealed several small-scale evolutionary rearrangements, in addition to providing evidence for the 4AL-5AL-7BS translocation in wild emmer wheat. Chromosome-based sequence assemblies contained five novel miRNA families, among 59 families putatively encoded in the entire genome which provide insight into the domestication of wheat and an overview of the genome content and organization.

Original languageEnglish
Pages (from-to)2077-2087
Number of pages11
JournalPlant Biotechnology Journal
Volume16
Issue number12
DOIs
StatePublished - Dec 2018
Externally publishedYes

Bibliographical note

Funding Information:
We greatly acknowledge the support from Montana Plant Sciences Endowment and Winifred-Asbjornson Plant Science award. We also thank Dr. István Molnár for providing Triticum

Funding Information:
dicoccoides seeds (accession MvGB436). We are grateful to Zden≤ka Dubská and Romana Sperkov≤ á for technical assistance with chromosome sorting. JV, MK and JD were supported by the Czech Science Foundation (award P501/12/G090) and by Ministry of Education, Youth and Sports of the Czech Republic (award LO1204 from the National Program of Sustainability I). PH was funded by grants AGL2016-77149-C2-1-P, and CGL2016-79790-P from MINECO, Spain and by grant P12-AGR-0482 from Junta de Andalucia, Spain.

Publisher Copyright:
© 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

Keywords

  • chromosome sorting
  • comparative genomics
  • hexaploid wheat
  • next-generation sequencing
  • wild emmer wheat

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

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