The objective of the present study was to map quantitative trait loci (QTL) controlling drought-resistance traits at seedling stage of Israeli wild barley (Hordeum spontaneum). Mapping was conducted using an F4 mapping population derived from a cross between genotype WQ23-38 from xeric (Wadi Qilt, 144 mm annual rainfall) and MA10-30 from mesic (Maalot, 790 mm annual rainfall) environments. Obvious segregation was observed for traits including time to wilt, leaf relative elongation rate, recovery rate, shoot regrowth, osmolarity, relative water content, leaf length, and root length with the coefficient of variation ranging from 10 to 77%. Eighteen putative QTL effects were identified (at false discovery rate = 10%) by single-trait analyses and confirmed by multi-trait analysis and multiple interval mapping using a new MultiQTL package. The results of our study suggest that the xeric genotypes adopted survival strategies while the mesic genotypes adopted growth-sustain strategies to cope with drought stress.
Bibliographical noteFunding Information:
Acknowledgments This work was supported by the following grants: German-Israeli Project Cooperation (grant DIP-B-4.3), the US AID Cooperative Development Research Program (grant TA-MOU-97_CA17-001), One Hundred Talents Project of The Chinese Academy of Sciences, the Israel Discount Bank Chair of Evolutionary Biology, the Ancell-Teicher Research Foundation for Molecular Genetics and Evolution, and the Graduate School of the University of Haifa, Israel. The authors thank Dr. Avigdor Beiles for valuable advice, Mrs. Robin Permut for editing, and Mrs. Ma Yan and Ms. Milade Naela for their field and experimental assistance.
- Drought resistance
- Genetic map
- Hordeum spontaneum
- QTL mapping
- Wild barley
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Agronomy and Crop Science
- Plant Science