Changes in Gene Expression in the Locus Coeruleus-Amygdala Circuitry in Inhibitory Avoidance PTSD Model

Esther L. Sabban, Lidia I. Serova, Elizabeth Newman, Nurit Aisenberg, Irit Akirav

Research output: Contribution to journalArticlepeer-review


The locus coeruleus (LC)-amygdala circuit is implicated in playing a key role in responses to emotionally arousing stimuli and in the manifestation of post-traumatic stress disorder (PTSD). Here, we examined changes in gene expression of a number of important mediators of the LC-amygdala circuitry in the inhibition avoidance model of PTSD. After testing for basal acoustic startle response (ASR), rats were exposed to a severe footshock (1.5 mA for 10 s) in the inhibitory avoidance apparatus. They were given contextual situational reminders every 5 day for 25 days. Controls were treated identically but with the footshock inactivated. Animals were re-tested on second ASR and decapitated 1 h later. The shock group had enhanced hyperarousal and several changes in gene expression compared to controls. In the LC, mRNA levels of norepinephrine (NE) biosynthetic enzymes (TH, DBH), NE transporter (NET), NPY receptors (Y1R, Y2R), and CB1 receptor of endocannabinoid system were elevated. In the basolateral amygdala (BLA), there were marked reductions in gene expression for CB1, and especially Y1R, with rise for corticotropin-releasing hormone (CRH) system (CRH, CRH receptor 1), and no significant changes in the central amygdala. Our results suggest a fast forward mechanism in the LC-amygdala circuitry in the shock group.

Original languageEnglish
Pages (from-to)273-280
Number of pages8
JournalCellular and Molecular Neurobiology
Issue number1
StatePublished - 1 Jan 2018

Bibliographical note

Publisher Copyright:
© 2017, Springer Science+Business Media, LLC.


  • Cannabinoid receptor
  • Corticotropin-releasing hormone
  • Neuropeptide Y
  • Neuropeptide Y1 and Y2 receptors
  • Norepinephrine transporter
  • Tyrosine hydroxylase

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology


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