Centrosomal Chk2 in DNA damage responses and cell cycle progession

Amnon Golan, Elah Pick, Lyuben Tsvetkov, Yasmine Nadler, Harriet Kluger, David F. Stern

Research output: Contribution to journalArticlepeer-review


Two major control systems regulate early stages of mitosis: activation of Cdk1 and anaphase control through assembly and disassembly of the mitotic spindle. In parallel to cell cycle progression, centrosomal duplication is regulated through proteins including Nek2. Recent studies suggest that centrosome-localized Chk1 forestalls premature activation of centrosomal Cdc25b and Cdk1 for mitotic entry, whereas Chk2 binds centrosomes and arrests mitosis only after activation by ATM and ATR in response to DNA damage. Here, we show that Chk2 centrosomal binding does not require DNA damage, but varies according to cell cycle progression. These and other data suggest a model in which binding of Chk2 to the centrosome at multiple cell cycle junctures controls co-localization of Chk2 with other cell cycle and centrosomal regulators.

Original languageEnglish
Pages (from-to)2647-2656
Number of pages10
JournalCell Cycle
Issue number13
StatePublished - 1 Jul 2010

Bibliographical note

Funding Information:
This work was supported by United States Army Medical Research and Materiel Command grant DAMD17-03-1-0331, and US PHS R01CA82257 to D.F.S.


  • Centrosome
  • Checkpoint
  • Chk2
  • DNA damage
  • Kinase-defective
  • Wild type

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology


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